Immune Repertoire Sequencing Using Molecular Identifiers Enables Accurate Clonality Discovery and Clone Size Quantification

Immune Repertoire Sequencing Using Molecular Identifiers Enables Accurate Clonality Discovery and Clone Size Quantification

Unique molecular identifiers (MIDs) have been demonstrated to effectively improve immune repertoire sequencing (IR-seq) accuracy, especially to identify somatic hypermutations in antibody repertoire sequencing. However, evaluating the sensitivity to detect rare T cells and the degree of clonal expan...

Full description

Bibliographic Details
Main Authors: Ke-Yue Ma, Chenfeng He, Ben S. Wendel, Chad M. Williams, Jun Xiao, Hui Yang, Ning Jiang
Format: Article
Language:English
Published: Frontiers Media S.A. 2018-02-01
Series:Frontiers in Immunology
Subjects:
MID clustering-based IR-Seq TCR repertoire sequencing
molecular identifiers
sub-clustering
naïve T cells
CMV-specific T cells
Online Access:http://journal.frontiersin.org/article/10.3389/fimmu.2018.00033/full

Internet

http://journal.frontiersin.org/article/10.3389/fimmu.2018.00033/full

Similar Items