Advanced Data Analysis for Fluorescence-Lifetime Single-Molecule Localization Microscopy

Advanced Data Analysis for Fluorescence-Lifetime Single-Molecule Localization Microscopy

Fluorescence-lifetime single molecule localization microscopy (FL-SMLM) adds the lifetime dimension to the spatial super-resolution provided by SMLM. Independent of intensity and spectrum, this lifetime information can be used, for example, to quantify the energy transfer efficiency in Förster Reson...

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Bibliographic Details
Main Authors: Jan Christoph Thiele, Oleksii Nevskyi, Dominic A. Helmerich, Markus Sauer, Jörg Enderlein
Format: Article
Language:English
Published: Frontiers Media S.A. 2021-11-01
Series:Frontiers in Bioinformatics
Subjects:
FLIM (fluorescence lifetime imaging microscopy)
CRLB (Cramér-Rao lower bound) analysis
fluoresence lifetime fitting
super-resolution microscopy
lifetime uncertainty
SMLM (single molecule localisation microscopy)
Online Access:https://www.frontiersin.org/articles/10.3389/fbinf.2021.740281/full

Internet

https://www.frontiersin.org/articles/10.3389/fbinf.2021.740281/full

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