Pollen-Specific CRISPR/Cas9 System to Increase Heritable Gene Mutations in Maize
The CRISPR/Cas9 system has been widely utilized in plant biotechnology as a gene editing tool. However, a conventional design with ubiquitously expressed CRISPR/Cas9 was observed to cause large numbers of somatic mutations that complicated the identification of heritable mutations. We constructed a...
Main Authors: | , , , , |
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Format: | Article |
Language: | English |
Published: |
MDPI AG
2021-08-01
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Series: | Agriculture |
Subjects: | |
Online Access: | https://www.mdpi.com/2077-0472/11/8/751 |