Pollen-Specific CRISPR/Cas9 System to Increase Heritable Gene Mutations in Maize

The CRISPR/Cas9 system has been widely utilized in plant biotechnology as a gene editing tool. However, a conventional design with ubiquitously expressed CRISPR/Cas9 was observed to cause large numbers of somatic mutations that complicated the identification of heritable mutations. We constructed a...

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Bibliographic Details
Main Authors: Changshuang Jing, Min Wei, Peng Fang, Rentao Song, Weiwei Qi
Format: Article
Language:English
Published: MDPI AG 2021-08-01
Series:Agriculture
Subjects:
Online Access:https://www.mdpi.com/2077-0472/11/8/751