Mycoprotein Production by Submerged Fermentation of the Edible Mushroom <i>Pleurotus ostreatus</i> in a Batch Stirred Tank Bioreactor Using Agro-Industrial Hydrolysate
The demand for cheap, healthy, and sustainable alternative protein sources has turned research interest into microbial proteins. Mycoproteins prevail due to their quite balanced amino acid profile, low carbon footprint and high sustainability potential. The goal of this research was to investigate t...
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| Format: | Article |
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MDPI AG
2023-06-01
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| Series: | Foods |
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| Online Access: | https://www.mdpi.com/2304-8158/12/12/2295 |
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| author | Georgios Bakratsas Angeliki Polydera Oskar Nilson Alexandra V. Chatzikonstantinou Charilaos Xiros Petros Katapodis Haralambos Stamatis |
| author_facet | Georgios Bakratsas Angeliki Polydera Oskar Nilson Alexandra V. Chatzikonstantinou Charilaos Xiros Petros Katapodis Haralambos Stamatis |
| author_sort | Georgios Bakratsas |
| collection | DOAJ |
| description | The demand for cheap, healthy, and sustainable alternative protein sources has turned research interest into microbial proteins. Mycoproteins prevail due to their quite balanced amino acid profile, low carbon footprint and high sustainability potential. The goal of this research was to investigate the capability of <i>Pleurotus ostreatus</i> to metabolize the main sugars of agro-industrial side streams, such as aspen wood chips hydrolysate, to produce high-value protein with low cost. Our results indicate that <i>P. ostreatus</i> LGAM 1123 could be cultivated both in a C-6 (glucose)- and C-5(xylose)-sugar-containing medium for mycoprotein production. A mixture of glucose and xylose was found to be ideal for biomass production with high protein content and rich amino acid profile. <i>P. ostreatus</i> LGAM 1123 cultivation in a 4 L stirred-tank bioreactor using aspen hydrolysate was achieved with 25.0 ± 3.4 g L<sup>−1</sup> biomass production, 1.8 ± 0.4 d<sup>−1</sup> specific growth rate and a protein yield of 54.5 ± 0.5% (g/100 g sugars). PCA analysis of the amino acids revealed a strong correlation between the amino acid composition of the protein produced and the ratios of glucose and xylose in the culture medium. The production of high-nutrient mycoprotein by submerged fermentation of the edible fungus <i>P. ostreatus</i> using agro-industrial hydrolysates is a promising bioprocess in the food and feed industry. |
| first_indexed | 2024-03-11T02:28:51Z |
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| id | doaj.art-84fd2de5bff240d99cfa9fd4ce406f3f |
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| language | English |
| last_indexed | 2024-03-11T02:28:51Z |
| publishDate | 2023-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Foods |
| spelling | doaj.art-84fd2de5bff240d99cfa9fd4ce406f3f2023-11-18T10:23:45ZengMDPI AGFoods2304-81582023-06-011212229510.3390/foods12122295Mycoprotein Production by Submerged Fermentation of the Edible Mushroom <i>Pleurotus ostreatus</i> in a Batch Stirred Tank Bioreactor Using Agro-Industrial HydrolysateGeorgios Bakratsas0Angeliki Polydera1Oskar Nilson2Alexandra V. Chatzikonstantinou3Charilaos Xiros4Petros Katapodis5Haralambos Stamatis6Biotechnology Laboratory, Department of Biological Applications and Technologies, University of Ioannina, 45110 Ioannina, GreeceBiotechnology Laboratory, Department of Biological Applications and Technologies, University of Ioannina, 45110 Ioannina, GreeceRISE Processum AB, SE-89122 Örnsköldsvik, SwedenBiotechnology Laboratory, Department of Biological Applications and Technologies, University of Ioannina, 45110 Ioannina, GreeceRISE Processum AB, SE-89122 Örnsköldsvik, SwedenBiotechnology Laboratory, Department of Biological Applications and Technologies, University of Ioannina, 45110 Ioannina, GreeceBiotechnology Laboratory, Department of Biological Applications and Technologies, University of Ioannina, 45110 Ioannina, GreeceThe demand for cheap, healthy, and sustainable alternative protein sources has turned research interest into microbial proteins. Mycoproteins prevail due to their quite balanced amino acid profile, low carbon footprint and high sustainability potential. The goal of this research was to investigate the capability of <i>Pleurotus ostreatus</i> to metabolize the main sugars of agro-industrial side streams, such as aspen wood chips hydrolysate, to produce high-value protein with low cost. Our results indicate that <i>P. ostreatus</i> LGAM 1123 could be cultivated both in a C-6 (glucose)- and C-5(xylose)-sugar-containing medium for mycoprotein production. A mixture of glucose and xylose was found to be ideal for biomass production with high protein content and rich amino acid profile. <i>P. ostreatus</i> LGAM 1123 cultivation in a 4 L stirred-tank bioreactor using aspen hydrolysate was achieved with 25.0 ± 3.4 g L<sup>−1</sup> biomass production, 1.8 ± 0.4 d<sup>−1</sup> specific growth rate and a protein yield of 54.5 ± 0.5% (g/100 g sugars). PCA analysis of the amino acids revealed a strong correlation between the amino acid composition of the protein produced and the ratios of glucose and xylose in the culture medium. The production of high-nutrient mycoprotein by submerged fermentation of the edible fungus <i>P. ostreatus</i> using agro-industrial hydrolysates is a promising bioprocess in the food and feed industry.https://www.mdpi.com/2304-8158/12/12/2295single-cell proteinsubmerged cultivationagro-industrial hydrolysateamino acid metabolismbioreactors |
| spellingShingle | Georgios Bakratsas Angeliki Polydera Oskar Nilson Alexandra V. Chatzikonstantinou Charilaos Xiros Petros Katapodis Haralambos Stamatis Mycoprotein Production by Submerged Fermentation of the Edible Mushroom <i>Pleurotus ostreatus</i> in a Batch Stirred Tank Bioreactor Using Agro-Industrial Hydrolysate Foods single-cell protein submerged cultivation agro-industrial hydrolysate amino acid metabolism bioreactors |
| title | Mycoprotein Production by Submerged Fermentation of the Edible Mushroom <i>Pleurotus ostreatus</i> in a Batch Stirred Tank Bioreactor Using Agro-Industrial Hydrolysate |
| title_full | Mycoprotein Production by Submerged Fermentation of the Edible Mushroom <i>Pleurotus ostreatus</i> in a Batch Stirred Tank Bioreactor Using Agro-Industrial Hydrolysate |
| title_fullStr | Mycoprotein Production by Submerged Fermentation of the Edible Mushroom <i>Pleurotus ostreatus</i> in a Batch Stirred Tank Bioreactor Using Agro-Industrial Hydrolysate |
| title_full_unstemmed | Mycoprotein Production by Submerged Fermentation of the Edible Mushroom <i>Pleurotus ostreatus</i> in a Batch Stirred Tank Bioreactor Using Agro-Industrial Hydrolysate |
| title_short | Mycoprotein Production by Submerged Fermentation of the Edible Mushroom <i>Pleurotus ostreatus</i> in a Batch Stirred Tank Bioreactor Using Agro-Industrial Hydrolysate |
| title_sort | mycoprotein production by submerged fermentation of the edible mushroom i pleurotus ostreatus i in a batch stirred tank bioreactor using agro industrial hydrolysate |
| topic | single-cell protein submerged cultivation agro-industrial hydrolysate amino acid metabolism bioreactors |
| url | https://www.mdpi.com/2304-8158/12/12/2295 |
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