Relocation of the attTn7 Transgene Insertion Site in Bacmid DNA Enhances Baculovirus Genome Stability and Recombinant Protein Expression in Insect Cells

Relocation of the attTn7 Transgene Insertion Site in Bacmid DNA Enhances Baculovirus Genome Stability and Recombinant Protein Expression in Insect Cells

Baculovirus expression vectors are successfully used for the commercial production of complex (glyco)proteins in eukaryotic cells. The genome engineering of single-copy baculovirus infectious clones (bacmids) in <i>E. coli</i> has been valuable in the study of baculovirus biology, but ba...

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Bibliographic Details
Main Authors: Gorben P. Pijlman, Carissa Grose, Tessy A. H. Hick, Herman E. Breukink, Robin van den Braak, Sandra R. Abbo, Corinne Geertsema, Monique M. van Oers, Dirk E. Martens, Dominic Esposito
Format: Article
Language:English
Published: MDPI AG 2020-12-01
Series:Viruses
Subjects:
baculovirus
insect cells
bacmid
Tn7
genome stability
protein expression
Online Access:https://www.mdpi.com/1999-4915/12/12/1448

Internet

https://www.mdpi.com/1999-4915/12/12/1448

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