Exosomal RNF157 mRNA from prostate cancer cells contributes to M2 macrophage polarization through destabilizing HDAC1
BackgroundExosomes have been identified to mediate the transmission of RNAs among different cells in tumor microenvironment, thus affecting the progression of different diseases. However, exosomal messenger RNAs (mRNAs) have been rarely explored. RNF157 mRNA has been found to be up-regulated in PCa...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-10-01
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| Series: | Frontiers in Oncology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fonc.2022.1021270/full |
| _version_ | 1818024421603934208 |
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| author | Han Guan Likai Mao Jinfeng Wang Sheng Wang Shuai Yang Hongliang Wu Wenyan Sun Zhijun Chen Ming Chen |
| author_facet | Han Guan Likai Mao Jinfeng Wang Sheng Wang Shuai Yang Hongliang Wu Wenyan Sun Zhijun Chen Ming Chen |
| author_sort | Han Guan |
| collection | DOAJ |
| description | BackgroundExosomes have been identified to mediate the transmission of RNAs among different cells in tumor microenvironment, thus affecting the progression of different diseases. However, exosomal messenger RNAs (mRNAs) have been rarely explored. RNF157 mRNA has been found to be up-regulated in PCa patients’ exosomes, but the role of exosomal RNF157 mRNA in PCa development remains unclear.MethodsOnline databases were utilized for predicting gene expression and binding correlation between different factors. RT-qPCR and western blot assays were respectively done to analyze RNA and protein expressions. Flow cytometry analysis was implemented to analyze M2 polarization.ResultsRNF157 expression was high in PCa tissues and cells. M2 polarization of macrophages was enhanced after co-culture with PCa cells or with exosomes released by PCa cells. Upon RNF157 knockdown in PCa cells, the extracted exosomes could not lead to the facilitated M2 polarization. Mechanistically, RNF157 could bind to HDAC1 and contribute to HDAC1 ubiquitination, which led to HDAC1 degradation and resulting in promoting M2 polarization of macrophages. Animal experiments validated that exosomal RNF157 accelerated PCa tumor growth through facilitating macrophage M2 polarization.ConclusionExosome-mediated RNF157 mRNA from PCa cells results in M2 macrophage polarization via destabilizing HDAC1, consequently promoting PCa tumor progression. |
| first_indexed | 2024-12-10T03:59:57Z |
| format | Article |
| id | doaj.art-850fc8d0d5f04c6cbd80a866d30bfc60 |
| institution | Directory Open Access Journal |
| issn | 2234-943X |
| language | English |
| last_indexed | 2024-12-10T03:59:57Z |
| publishDate | 2022-10-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Oncology |
| spelling | doaj.art-850fc8d0d5f04c6cbd80a866d30bfc602022-12-22T02:02:59ZengFrontiers Media S.A.Frontiers in Oncology2234-943X2022-10-011210.3389/fonc.2022.10212701021270Exosomal RNF157 mRNA from prostate cancer cells contributes to M2 macrophage polarization through destabilizing HDAC1Han Guan0Likai Mao1Jinfeng Wang2Sheng Wang3Shuai Yang4Hongliang Wu5Wenyan Sun6Zhijun Chen7Ming Chen8Department of Urology, The First Affiliated Hospital of Bengbu Medical College, Bengbu, ChinaDepartment of Urology, The Second Affiliated Hospital of Bengbu Medical College, Bengbu, ChinaDepartment of Urology, Yancheng No. 3 People’s Hospital, Yancheng, ChinaDepartment of Urology, The First Affiliated Hospital of Bengbu Medical College, Bengbu, ChinaDepartment of Urology, The First Affiliated Hospital of Bengbu Medical College, Bengbu, ChinaDepartment of Urology, The First Affiliated Hospital of Bengbu Medical College, Bengbu, ChinaDepartment of Urology, The First Affiliated Hospital of Bengbu Medical College, Bengbu, ChinaDepartment of Urology, The First Affiliated Hospital of Bengbu Medical College, Bengbu, ChinaDepartment of Urology, Zhongda Hospital Affiliated to Southeast University, Nanjing, ChinaBackgroundExosomes have been identified to mediate the transmission of RNAs among different cells in tumor microenvironment, thus affecting the progression of different diseases. However, exosomal messenger RNAs (mRNAs) have been rarely explored. RNF157 mRNA has been found to be up-regulated in PCa patients’ exosomes, but the role of exosomal RNF157 mRNA in PCa development remains unclear.MethodsOnline databases were utilized for predicting gene expression and binding correlation between different factors. RT-qPCR and western blot assays were respectively done to analyze RNA and protein expressions. Flow cytometry analysis was implemented to analyze M2 polarization.ResultsRNF157 expression was high in PCa tissues and cells. M2 polarization of macrophages was enhanced after co-culture with PCa cells or with exosomes released by PCa cells. Upon RNF157 knockdown in PCa cells, the extracted exosomes could not lead to the facilitated M2 polarization. Mechanistically, RNF157 could bind to HDAC1 and contribute to HDAC1 ubiquitination, which led to HDAC1 degradation and resulting in promoting M2 polarization of macrophages. Animal experiments validated that exosomal RNF157 accelerated PCa tumor growth through facilitating macrophage M2 polarization.ConclusionExosome-mediated RNF157 mRNA from PCa cells results in M2 macrophage polarization via destabilizing HDAC1, consequently promoting PCa tumor progression.https://www.frontiersin.org/articles/10.3389/fonc.2022.1021270/fullexosomal RNF157 mRNAprostate cancerM2 macrophage polarizationHDAC1ubiquitination |
| spellingShingle | Han Guan Likai Mao Jinfeng Wang Sheng Wang Shuai Yang Hongliang Wu Wenyan Sun Zhijun Chen Ming Chen Exosomal RNF157 mRNA from prostate cancer cells contributes to M2 macrophage polarization through destabilizing HDAC1 Frontiers in Oncology exosomal RNF157 mRNA prostate cancer M2 macrophage polarization HDAC1 ubiquitination |
| title | Exosomal RNF157 mRNA from prostate cancer cells contributes to M2 macrophage polarization through destabilizing HDAC1 |
| title_full | Exosomal RNF157 mRNA from prostate cancer cells contributes to M2 macrophage polarization through destabilizing HDAC1 |
| title_fullStr | Exosomal RNF157 mRNA from prostate cancer cells contributes to M2 macrophage polarization through destabilizing HDAC1 |
| title_full_unstemmed | Exosomal RNF157 mRNA from prostate cancer cells contributes to M2 macrophage polarization through destabilizing HDAC1 |
| title_short | Exosomal RNF157 mRNA from prostate cancer cells contributes to M2 macrophage polarization through destabilizing HDAC1 |
| title_sort | exosomal rnf157 mrna from prostate cancer cells contributes to m2 macrophage polarization through destabilizing hdac1 |
| topic | exosomal RNF157 mRNA prostate cancer M2 macrophage polarization HDAC1 ubiquitination |
| url | https://www.frontiersin.org/articles/10.3389/fonc.2022.1021270/full |
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