Combining RNAscope and immunohistochemistry to visualize inflammatory gene products in neurons and microglia

Combining RNAscope and immunohistochemistry to visualize inflammatory gene products in neurons and microglia

A challenge for central nervous system (CNS) tissue analysis in neuroscience research has been the difficulty to codetect and colocalize gene and protein expression in the same tissue. Given the importance of identifying gene expression relative to proteins of interest, for example, cell-type specif...

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Bibliographic Details
Main Authors: Jayson B. Ball, Connor J. McNulty, Suzanne M. Green-Fulgham, Joseph M. Dragavon, Igor R. Correia Rocha, Maggie R. Finch, Emily D. Prévost, Imaad I. Siddique, Brodie J. Woodall, Linda R. Watkins, Michael V. Baratta, David H. Root
Format: Article
Language:English
Published: Frontiers Media S.A. 2023-08-01
Series:Frontiers in Molecular Neuroscience
Subjects:
hybridization
spinal cord
neuroinflammation
NLRP3
interleukin-1beta
Imaris
Online Access:https://www.frontiersin.org/articles/10.3389/fnmol.2023.1225847/full

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https://www.frontiersin.org/articles/10.3389/fnmol.2023.1225847/full

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