Genome editing by introduction of Cas9/sgRNA into plant cells using temperature-controlled atmospheric pressure plasma.

Genome editing by introduction of Cas9/sgRNA into plant cells using temperature-controlled atmospheric pressure plasma.

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Previously, we developed a technique to introduce a superfolder green fluorescent protein (sGFP) fusion protein directly into plant cells using atmospheric-pressure plasma. In this study, we attempted genome editing using CRISPR/Cas9 (clustered regularly interspaced short palindromic repeats/CRISPR...

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Bibliographic Details
Main Authors: Yuki Yanagawa, Yuma Suenaga, Yusuke Iijima, Masaki Endo, Naoko Sanada, Etsuko Katoh, Seiichi Toki, Akitoshi Okino, Ichiro Mitsuhara
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2023-01-01
Series:PLoS ONE
Online Access:https://doi.org/10.1371/journal.pone.0281767

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https://doi.org/10.1371/journal.pone.0281767

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