De novo formation of left-right asymmetry by posterior tilt of nodal cilia.
In the developing mouse embryo, leftward fluid flow on the ventral side of the node determines left-right (L-R) asymmetry. However, the mechanism by which the rotational movement of node cilia can generate a unidirectional flow remains hypothetical. Here we have addressed this question by motion and...
| Format: | Article |
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| Language: | English |
| Published: |
Public Library of Science (PLoS)
2005-08-01
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| Series: | PLoS Biology |
| Online Access: | http://dx.doi.org/10.1371/journal.pbio.0030268 |
| _version_ | 1818433781332180992 |
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| collection | DOAJ |
| description | In the developing mouse embryo, leftward fluid flow on the ventral side of the node determines left-right (L-R) asymmetry. However, the mechanism by which the rotational movement of node cilia can generate a unidirectional flow remains hypothetical. Here we have addressed this question by motion and morphological analyses of the node cilia and by fluid dynamic model experiments. We found that the cilia stand, not perpendicular to the node surface, but tilted posteriorly. We further confirmed that such posterior tilt can produce leftward flow in model experiments. These results strongly suggest that L-R asymmetry is not the descendant of pre-existing L-R asymmetry within each cell but is generated de novo by combining three sources of spatial information: antero-posterior and dorso-ventral axes, and the chirality of ciliary movement. |
| first_indexed | 2024-12-14T16:26:33Z |
| format | Article |
| id | doaj.art-855fc9abec834541ae5c555ba26af6c8 |
| institution | Directory Open Access Journal |
| issn | 1544-9173 1545-7885 |
| language | English |
| last_indexed | 2024-12-14T16:26:33Z |
| publishDate | 2005-08-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Biology |
| spelling | doaj.art-855fc9abec834541ae5c555ba26af6c82022-12-21T22:54:41ZengPublic Library of Science (PLoS)PLoS Biology1544-91731545-78852005-08-0138e268De novo formation of left-right asymmetry by posterior tilt of nodal cilia.In the developing mouse embryo, leftward fluid flow on the ventral side of the node determines left-right (L-R) asymmetry. However, the mechanism by which the rotational movement of node cilia can generate a unidirectional flow remains hypothetical. Here we have addressed this question by motion and morphological analyses of the node cilia and by fluid dynamic model experiments. We found that the cilia stand, not perpendicular to the node surface, but tilted posteriorly. We further confirmed that such posterior tilt can produce leftward flow in model experiments. These results strongly suggest that L-R asymmetry is not the descendant of pre-existing L-R asymmetry within each cell but is generated de novo by combining three sources of spatial information: antero-posterior and dorso-ventral axes, and the chirality of ciliary movement.http://dx.doi.org/10.1371/journal.pbio.0030268 |
| spellingShingle | De novo formation of left-right asymmetry by posterior tilt of nodal cilia. PLoS Biology |
| title | De novo formation of left-right asymmetry by posterior tilt of nodal cilia. |
| title_full | De novo formation of left-right asymmetry by posterior tilt of nodal cilia. |
| title_fullStr | De novo formation of left-right asymmetry by posterior tilt of nodal cilia. |
| title_full_unstemmed | De novo formation of left-right asymmetry by posterior tilt of nodal cilia. |
| title_short | De novo formation of left-right asymmetry by posterior tilt of nodal cilia. |
| title_sort | de novo formation of left right asymmetry by posterior tilt of nodal cilia |
| url | http://dx.doi.org/10.1371/journal.pbio.0030268 |