Hibiscus sabdariffa synthesized gold nanoparticles ameliorate aluminum chloride induced memory deficits through inhibition of COX-2/BACE-1 mRNA expression in rats
Alzheimer’s disease (AD) is a major health challenge worldwide, especially among the elderly. The disease is associated with cognitive and memory deficits. This study investigated the effect of Hibiscus sabdariffa synthesized-gold nanoparticles (HS-AuNPs) on AlCl3-induced memory deficits in rats. Fo...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-04-01
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| Series: | Arabian Journal of Chemistry |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1878535223000655 |
| _version_ | 1811159976395145216 |
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| author | Scholastica O. Anadozie Duncan O. Effiom Olusola B. Adewale Jodie Jude Itumeleng Zosela Oluwole B. Akawa Juliet N. Olayinka Saartjie Roux |
| author_facet | Scholastica O. Anadozie Duncan O. Effiom Olusola B. Adewale Jodie Jude Itumeleng Zosela Oluwole B. Akawa Juliet N. Olayinka Saartjie Roux |
| author_sort | Scholastica O. Anadozie |
| collection | DOAJ |
| description | Alzheimer’s disease (AD) is a major health challenge worldwide, especially among the elderly. The disease is associated with cognitive and memory deficits. This study investigated the effect of Hibiscus sabdariffa synthesized-gold nanoparticles (HS-AuNPs) on AlCl3-induced memory deficits in rats. Forty-two male Wistar rats were divided into six groups (n = 7). Group I served as control. Rats in group II - V were exposed to AlCl3 (100 mg/kg) to induce AD. Group III - V rats were treated with 5 mg/kg donepezil, 5 and 10 mg/kg HS-AuNPs, respectively, for 14 days. Behavioral tests were carried out on the rats on day 28 and 42. At the end of animal experiment, rats were sacrificed and used for various biochemical assays and gene expression. The AD rats showed memory and learning impairment, and these conditions were ameliorated by HS-AuNPs. Significant (p < 0.05) elevation in the activities of acetylcholinesterase, monoamine oxidase and adenosine deaminase, as well as malondialdehyde levels was noted. A significant reduction in the activities of superoxide dismutase (SOD), glutathione peroxidase (GPx) and reduced glutathione (GSH) noted in AlCl3-induced rats were ameliorated by the 5 and 10 mg/kg b.w. doses of HS-AuNPs. In addition, the increased mRNA expression of cyclooxygenase-2 (COX-2) and beta-secretase 1 (BACE-1) caused by AlCl3 were assuaged by the HS-AuNPs treatment. Based on the activities of HS-AuNPs against AlCl3-induced AD, HS-AuNPs could be considered a potential therapeutic agent for managing AD. |
| first_indexed | 2024-04-10T05:49:51Z |
| format | Article |
| id | doaj.art-857876a0a38d4976b7896bc34a26bb63 |
| institution | Directory Open Access Journal |
| issn | 1878-5352 |
| language | English |
| last_indexed | 2024-04-10T05:49:51Z |
| publishDate | 2023-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Arabian Journal of Chemistry |
| spelling | doaj.art-857876a0a38d4976b7896bc34a26bb632023-03-05T04:24:00ZengElsevierArabian Journal of Chemistry1878-53522023-04-01164104604Hibiscus sabdariffa synthesized gold nanoparticles ameliorate aluminum chloride induced memory deficits through inhibition of COX-2/BACE-1 mRNA expression in ratsScholastica O. Anadozie0Duncan O. Effiom1Olusola B. Adewale2Jodie Jude3Itumeleng Zosela4Oluwole B. Akawa5Juliet N. Olayinka6Saartjie Roux7Biochemistry Program, Department of Chemical Sciences, Afe Babalola University, P.M.B 5454, Ado-Ekiti, Nigeria; Corresponding author.Biochemistry Program, Department of Chemical Sciences, Afe Babalola University, P.M.B 5454, Ado-Ekiti, NigeriaBiochemistry Program, Department of Chemical Sciences, Afe Babalola University, P.M.B 5454, Ado-Ekiti, NigeriaBiochemistry Program, Department of Chemical Sciences, Afe Babalola University, P.M.B 5454, Ado-Ekiti, NigeriaDepartment of Human Physiology, Nelson Mandela University, P.O Box 77000, Port Elizabeth, South AfricaDepartment of Pharmacology and Toxicology, College of Pharmacy, Afe Babalola University, P.M.B 5454, Ado-Ekiti, Nigeria; Molecular Biocomputation and Drug Design Laboratory, School of Health Sciences, University of KwaZulu-Natal, Westville Campus, Durban 4001, South AfricaDepartment of Pharmacology and Therapeutics, College of Medicine and Health Sciences, Afe Babalola University, P.M.B 5454, Ado-Ekiti, NigeriaDepartment of Human Physiology, Nelson Mandela University, P.O Box 77000, Port Elizabeth, South AfricaAlzheimer’s disease (AD) is a major health challenge worldwide, especially among the elderly. The disease is associated with cognitive and memory deficits. This study investigated the effect of Hibiscus sabdariffa synthesized-gold nanoparticles (HS-AuNPs) on AlCl3-induced memory deficits in rats. Forty-two male Wistar rats were divided into six groups (n = 7). Group I served as control. Rats in group II - V were exposed to AlCl3 (100 mg/kg) to induce AD. Group III - V rats were treated with 5 mg/kg donepezil, 5 and 10 mg/kg HS-AuNPs, respectively, for 14 days. Behavioral tests were carried out on the rats on day 28 and 42. At the end of animal experiment, rats were sacrificed and used for various biochemical assays and gene expression. The AD rats showed memory and learning impairment, and these conditions were ameliorated by HS-AuNPs. Significant (p < 0.05) elevation in the activities of acetylcholinesterase, monoamine oxidase and adenosine deaminase, as well as malondialdehyde levels was noted. A significant reduction in the activities of superoxide dismutase (SOD), glutathione peroxidase (GPx) and reduced glutathione (GSH) noted in AlCl3-induced rats were ameliorated by the 5 and 10 mg/kg b.w. doses of HS-AuNPs. In addition, the increased mRNA expression of cyclooxygenase-2 (COX-2) and beta-secretase 1 (BACE-1) caused by AlCl3 were assuaged by the HS-AuNPs treatment. Based on the activities of HS-AuNPs against AlCl3-induced AD, HS-AuNPs could be considered a potential therapeutic agent for managing AD.http://www.sciencedirect.com/science/article/pii/S1878535223000655Aluminum chlorideAlzheimer’s diseaseCyclooxygenase-2Hibiscus sabdariffaGold nanoparticle |
| spellingShingle | Scholastica O. Anadozie Duncan O. Effiom Olusola B. Adewale Jodie Jude Itumeleng Zosela Oluwole B. Akawa Juliet N. Olayinka Saartjie Roux Hibiscus sabdariffa synthesized gold nanoparticles ameliorate aluminum chloride induced memory deficits through inhibition of COX-2/BACE-1 mRNA expression in rats Arabian Journal of Chemistry Aluminum chloride Alzheimer’s disease Cyclooxygenase-2 Hibiscus sabdariffa Gold nanoparticle |
| title | Hibiscus sabdariffa synthesized gold nanoparticles ameliorate aluminum chloride induced memory deficits through inhibition of COX-2/BACE-1 mRNA expression in rats |
| title_full | Hibiscus sabdariffa synthesized gold nanoparticles ameliorate aluminum chloride induced memory deficits through inhibition of COX-2/BACE-1 mRNA expression in rats |
| title_fullStr | Hibiscus sabdariffa synthesized gold nanoparticles ameliorate aluminum chloride induced memory deficits through inhibition of COX-2/BACE-1 mRNA expression in rats |
| title_full_unstemmed | Hibiscus sabdariffa synthesized gold nanoparticles ameliorate aluminum chloride induced memory deficits through inhibition of COX-2/BACE-1 mRNA expression in rats |
| title_short | Hibiscus sabdariffa synthesized gold nanoparticles ameliorate aluminum chloride induced memory deficits through inhibition of COX-2/BACE-1 mRNA expression in rats |
| title_sort | hibiscus sabdariffa synthesized gold nanoparticles ameliorate aluminum chloride induced memory deficits through inhibition of cox 2 bace 1 mrna expression in rats |
| topic | Aluminum chloride Alzheimer’s disease Cyclooxygenase-2 Hibiscus sabdariffa Gold nanoparticle |
| url | http://www.sciencedirect.com/science/article/pii/S1878535223000655 |
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