In situ FRET measurement of cellular tension using conventional confocal laser microscopy in newly established reporter mice expressing actinin tension sensor

Abstract FRET-based sensors are utilized for real-time measurements of cellular tension. However, transfection of the sensor gene shows low efficacy and is only effective for a short period. Reporter mice expressing such sensors have been developed, but sensor fluorescence has not been measured succ...

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Bibliographic Details
Main Authors: Junfeng Wang, Eijiro Maeda, Yuki Tsujimura, Takaya Abe, Hiroshi Kiyonari, Tetsuya Kitaguchi, Hideo Yokota, Takeo Matsumoto
Format: Article
Language:English
Published: Nature Portfolio 2023-12-01
Series:Scientific Reports
Online Access:https://doi.org/10.1038/s41598-023-50142-z