Highly efficient construction of infectious viroid-derived clones
Abstract Background Viroid research generally relies on infectious cDNA clones that consist of dimers of the entire viroid sequence. At present, those dimers are generated by self-ligation of monomeric cDNA, a strategy that presents several disadvantages: (i) low efficiency, (ii) it is a non-oriente...
Main Authors: | , , , |
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Format: | Article |
Language: | English |
Published: |
BMC
2019-08-01
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Series: | Plant Methods |
Subjects: | |
Online Access: | http://link.springer.com/article/10.1186/s13007-019-0470-4 |