Dual indexed library design enables compatibility of in-Drop single-cell RNA-sequencing with exAMP chemistry sequencing platforms

Dual indexed library design enables compatibility of in-Drop single-cell RNA-sequencing with exAMP chemistry sequencing platforms

Abstract Background The increasing demand of single-cell RNA-sequencing (scRNA-seq) experiments, such as the number of experiments and cells queried per experiment, necessitates higher sequencing depth coupled to high data quality. New high-throughput sequencers, such as the Illumina NovaSeq 6000, e...

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Bibliographic Details
Main Authors: Austin N. Southard-Smith, Alan J. Simmons, Bob Chen, Angela L. Jones, Marisol A. Ramirez Solano, Paige N. Vega, Cherie’ R. Scurrah, Yue Zhao, Michael J. Brenan, Jiekun Xuan, Martha J. Shrubsole, Ely B. Porter, Xi Chen, Colin J. H. Brenan, Qi Liu, Lauren N. M. Quigley, Ken S. Lau
Format: Article
Language:English
Published: BMC 2020-07-01
Series:BMC Genomics
Subjects:
Single-cell RNA sequencing
inDrop
TruSeq
Next-generation sequencing
NovaSeq
Index hopping
Online Access:http://link.springer.com/article/10.1186/s12864-020-06843-0

Internet

http://link.springer.com/article/10.1186/s12864-020-06843-0

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