A Novel and Quantitative Detection Assay (<i>effluxR</i>) for Identifying Efflux-Associated Resistance Genes Using Multiplex Digital PCR in Clinical Isolates of <i>Pseudomonas aeruginosa</i>

A Novel and Quantitative Detection Assay (<i>effluxR</i>) for Identifying Efflux-Associated Resistance Genes Using Multiplex Digital PCR in Clinical Isolates of <i>Pseudomonas aeruginosa</i>

The rise of multidrug resistance of <i>Pseudomonas aeruginosa</i> highlights an increased need for selective and precise antimicrobial treatment. Drug efflux pumps are one of the major mechanisms of antimicrobial resistance found in many bacteria, including <i>P</i>. <i>...

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Bibliographic Details
Main Authors: Nontaporn Rattanachak, Sattaporn Weawsiangsang, Robert A. Baldock, Theerasak Jaifoo, Touchkanin Jongjitvimol, Jirapas Jongjitwimol
Format: Article
Language:English
Published: MDPI AG 2023-10-01
Series:Methods and Protocols
Subjects:
<i>Pseudomonas aeruginosa</i>
antibiotic resistant genes
RND-type efflux pumps
multiplex digital polymerase chain reaction (mdPCR)
multiplex quantitative polymerase chain reaction (mqPCR)
Online Access:https://www.mdpi.com/2409-9279/6/5/96

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https://www.mdpi.com/2409-9279/6/5/96

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