Protocol to assess substrate dephosphorylation by serine/threonine phosphoprotein phosphatases in vitro

Protocol to assess substrate dephosphorylation by serine/threonine phosphoprotein phosphatases in vitro

Summary: Serine/threonine protein phosphatase 2 (PP2A) forms heterotrimeric holoenzymes, where a scaffold subunit bridges the PP2A catalytic subunit to a B regulatory subunit, e.g., B55α. The PP2A/B55α holoenzyme plays key roles in signaling and cell-cycle control targeting multiple substrates. Here...

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Bibliographic Details
Main Authors: Jason S. Wasserman, Felicity Feiser, Seren Palacio, Kishan Patel, Joy Gonzalez, Holly Fowle, Xavier Graña
Format: Article
Language:English
Published: Elsevier 2023-06-01
Series:STAR Protocols
Subjects:
Cell Culture
Chemistry
Molecular Biology
Protein Biochemistry
Protein Expression and Purification
Signal Transduction
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166723001065

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http://www.sciencedirect.com/science/article/pii/S2666166723001065

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