Detection of tuberculosis drug resistance: a comparison by Mycobacterium tuberculosis MLPA assay versus Genotype®MTBDRplus
BACKGROUND To cope with the emergence of multidrug-resistant tuberculosis (MDR-TB), new molecular methods that can routinely be used to screen for a wide range of drug resistance related genetic markers in the Mycobacterium tuberculosis genome are urgently needed. OBJECTIVE To evaluate the perfor...
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| Format: | Article |
| Language: | English |
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Fundação Oswaldo Cruz (FIOCRUZ)
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| Series: | Memorias do Instituto Oswaldo Cruz |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762017000600396&lng=en&tlng=en |
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| author | Paula Fernanda Gonçalves dos Santos Elis Regina Dalla Costa Daniela M Ramalho Maria Lucia Rossetti Regina Bones Barcellos Luciana de Souza Nunes Leonardo Souza Esteves Rodrigo Rodenbusch Richard M Anthony Indra Bergval Sarah Sengstake Miguel Viveiros Afrânio Kritski Martha M Oliveira |
| author_facet | Paula Fernanda Gonçalves dos Santos Elis Regina Dalla Costa Daniela M Ramalho Maria Lucia Rossetti Regina Bones Barcellos Luciana de Souza Nunes Leonardo Souza Esteves Rodrigo Rodenbusch Richard M Anthony Indra Bergval Sarah Sengstake Miguel Viveiros Afrânio Kritski Martha M Oliveira |
| author_sort | Paula Fernanda Gonçalves dos Santos |
| collection | DOAJ |
| description | BACKGROUND To cope with the emergence of multidrug-resistant tuberculosis (MDR-TB), new molecular methods that can routinely be used to screen for a wide range of drug resistance related genetic markers in the Mycobacterium tuberculosis genome are urgently needed. OBJECTIVE To evaluate the performance of multiplex ligaton-dependent probe amplification (MLPA) against Genotype® MTBDRplus to detect resistance to isoniazid (INHr) and rifampicin (RIFr). METHOD 96 culture isolates characterised for identification, drug susceptibility testing (DST) and sequencing of rpoB, katG, and inhA genes were evaluated by the MLPA and Genotype®MTBDRplus assays. RESULTS With sequencing as a reference standard, sensitivity (SE) to detect INHr was 92.8% and 85.7%, and specificity (SP) was 100% and 97.5%, for MLPA and Genotype®MTBDRplus, respectively. In relation to RIFr, SE was 87.5% and 100%, and SP was 100% and 98.8%, respectively. Kappa value was identical between Genotype®MTBDRplus and MLPA compared with the standard DST and sequencing for detection of INHr [0.83 (0.75-0.91)] and RIFr [0.93 (0.88-0.98)]. CONCLUSION Compared to Genotype®MTBDRplus, MLPA showed similar sensitivity to detect INH and RIF resistance. The results obtained by the MLPA and Genotype®MTBDRplus assays indicate that both molecular tests can be used for the rapid detection of drug-resistant TB with high accuracy. MLPA has the added value of providing information on the circulating M. tuberculosis lineages. |
| first_indexed | 2024-03-12T08:12:02Z |
| format | Article |
| id | doaj.art-86356cccc5134c48a540e1f17a46aa10 |
| institution | Directory Open Access Journal |
| issn | 1678-8060 |
| language | English |
| last_indexed | 2024-03-12T08:12:02Z |
| publisher | Fundação Oswaldo Cruz (FIOCRUZ) |
| record_format | Article |
| series | Memorias do Instituto Oswaldo Cruz |
| spelling | doaj.art-86356cccc5134c48a540e1f17a46aa102023-09-02T19:06:43ZengFundação Oswaldo Cruz (FIOCRUZ)Memorias do Instituto Oswaldo Cruz1678-8060112639640310.1590/0074-02760160376S0074-02762017000600396Detection of tuberculosis drug resistance: a comparison by Mycobacterium tuberculosis MLPA assay versus Genotype®MTBDRplusPaula Fernanda Gonçalves dos SantosElis Regina Dalla CostaDaniela M RamalhoMaria Lucia RossettiRegina Bones BarcellosLuciana de Souza NunesLeonardo Souza EstevesRodrigo RodenbuschRichard M AnthonyIndra BergvalSarah SengstakeMiguel ViveirosAfrânio KritskiMartha M OliveiraBACKGROUND To cope with the emergence of multidrug-resistant tuberculosis (MDR-TB), new molecular methods that can routinely be used to screen for a wide range of drug resistance related genetic markers in the Mycobacterium tuberculosis genome are urgently needed. OBJECTIVE To evaluate the performance of multiplex ligaton-dependent probe amplification (MLPA) against Genotype® MTBDRplus to detect resistance to isoniazid (INHr) and rifampicin (RIFr). METHOD 96 culture isolates characterised for identification, drug susceptibility testing (DST) and sequencing of rpoB, katG, and inhA genes were evaluated by the MLPA and Genotype®MTBDRplus assays. RESULTS With sequencing as a reference standard, sensitivity (SE) to detect INHr was 92.8% and 85.7%, and specificity (SP) was 100% and 97.5%, for MLPA and Genotype®MTBDRplus, respectively. In relation to RIFr, SE was 87.5% and 100%, and SP was 100% and 98.8%, respectively. Kappa value was identical between Genotype®MTBDRplus and MLPA compared with the standard DST and sequencing for detection of INHr [0.83 (0.75-0.91)] and RIFr [0.93 (0.88-0.98)]. CONCLUSION Compared to Genotype®MTBDRplus, MLPA showed similar sensitivity to detect INH and RIF resistance. The results obtained by the MLPA and Genotype®MTBDRplus assays indicate that both molecular tests can be used for the rapid detection of drug-resistant TB with high accuracy. MLPA has the added value of providing information on the circulating M. tuberculosis lineages.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762017000600396&lng=en&tlng=entuberculosisMLPAGenoTypeMTBDR®plusisoniazid resistancerifampicin resistancemultidrug resistance |
| spellingShingle | Paula Fernanda Gonçalves dos Santos Elis Regina Dalla Costa Daniela M Ramalho Maria Lucia Rossetti Regina Bones Barcellos Luciana de Souza Nunes Leonardo Souza Esteves Rodrigo Rodenbusch Richard M Anthony Indra Bergval Sarah Sengstake Miguel Viveiros Afrânio Kritski Martha M Oliveira Detection of tuberculosis drug resistance: a comparison by Mycobacterium tuberculosis MLPA assay versus Genotype®MTBDRplus Memorias do Instituto Oswaldo Cruz tuberculosis MLPA GenoTypeMTBDR®plus isoniazid resistance rifampicin resistance multidrug resistance |
| title | Detection of tuberculosis drug resistance: a comparison by Mycobacterium tuberculosis MLPA assay versus Genotype®MTBDRplus |
| title_full | Detection of tuberculosis drug resistance: a comparison by Mycobacterium tuberculosis MLPA assay versus Genotype®MTBDRplus |
| title_fullStr | Detection of tuberculosis drug resistance: a comparison by Mycobacterium tuberculosis MLPA assay versus Genotype®MTBDRplus |
| title_full_unstemmed | Detection of tuberculosis drug resistance: a comparison by Mycobacterium tuberculosis MLPA assay versus Genotype®MTBDRplus |
| title_short | Detection of tuberculosis drug resistance: a comparison by Mycobacterium tuberculosis MLPA assay versus Genotype®MTBDRplus |
| title_sort | detection of tuberculosis drug resistance a comparison by mycobacterium tuberculosis mlpa assay versus genotype r mtbdrplus |
| topic | tuberculosis MLPA GenoTypeMTBDR®plus isoniazid resistance rifampicin resistance multidrug resistance |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762017000600396&lng=en&tlng=en |
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