AIV polyantigen epitope expressed by recombinant baculovirus induces a systemic immune response in chicken and mouse models
Abstract Background The protective efficacy of avian influenza virus (AIV) vaccines is unsatisfactory due to the presence of various serotypes generated by genetic reassortment. Thus, immunization with a polyantigen chimeric epitope vaccine may be an effective strategy for protecting poultry from in...
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| Language: | English |
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BMC
2020-08-01
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| Series: | Virology Journal |
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| Online Access: | http://link.springer.com/article/10.1186/s12985-020-01388-w |
| _version_ | 1818279740741517312 |
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| author | Lei Yu Jun Pan Guangli Cao Mengsheng Jiang Yunshan Zhang Min Zhu Zi Liang Xing Zhang Xiaolong Hu Renyu Xue Chengliang Gong |
| author_facet | Lei Yu Jun Pan Guangli Cao Mengsheng Jiang Yunshan Zhang Min Zhu Zi Liang Xing Zhang Xiaolong Hu Renyu Xue Chengliang Gong |
| author_sort | Lei Yu |
| collection | DOAJ |
| description | Abstract Background The protective efficacy of avian influenza virus (AIV) vaccines is unsatisfactory due to the presence of various serotypes generated by genetic reassortment. Thus, immunization with a polyantigen chimeric epitope vaccine may be an effective strategy for protecting poultry from infection with different AIV subtypes. Methods Baculovirus has recently emerged as a novel and attractive gene delivery vehicle for animal cells. In the present study, a recombinant baculovirus BmNPV-CMV/THB-P10/CTLT containing a fused codon-optimized sequence (CTLT) of T lymphocyte epitopes from H1HA, H9HA, and H7HA AIV subtypes, and another fused codon-optimized sequence (THB) of Th and B cell epitopes from H1HA, H9HA, and H7HA AIV subtypes, driven by a baculovirus P10 promoter and cytomegalovirus CMV promoter, respectively, was constructed. Results Western blotting and cellular immunofluorescence demonstrated that the CTLT (THB) can be expressed in rBac-CMV/THB-P10/CTLT-infected silkworm cells (mammalian HEK293T cells). Furthermore, the recombinant virus, rBac-CMV-THB-CTLT, was used to immunize both chickens and mice. Conclusions The results of an indirect ELISA, immunohistochemistry, and T lymphocyte proliferation assay indicated that specific humoral and cellular responses were detected in both chicken and mice. These results suggest that rBac-CMV/THB-P10/CTLT can be developed as a potential vaccine against different AIV subtypes. |
| first_indexed | 2024-12-12T23:38:08Z |
| format | Article |
| id | doaj.art-8635adc08bf94568b757aa1a2f9c6ae6 |
| institution | Directory Open Access Journal |
| issn | 1743-422X |
| language | English |
| last_indexed | 2024-12-12T23:38:08Z |
| publishDate | 2020-08-01 |
| publisher | BMC |
| record_format | Article |
| series | Virology Journal |
| spelling | doaj.art-8635adc08bf94568b757aa1a2f9c6ae62022-12-22T00:07:19ZengBMCVirology Journal1743-422X2020-08-0117111310.1186/s12985-020-01388-wAIV polyantigen epitope expressed by recombinant baculovirus induces a systemic immune response in chicken and mouse modelsLei Yu0Jun Pan1Guangli Cao2Mengsheng Jiang3Yunshan Zhang4Min Zhu5Zi Liang6Xing Zhang7Xiaolong Hu8Renyu Xue9Chengliang Gong10School of Biology and Basic Medical Sciences, Soochow UniversitySchool of Biology and Basic Medical Sciences, Soochow UniversitySchool of Biology and Basic Medical Sciences, Soochow UniversitySchool of Biology and Basic Medical Sciences, Soochow UniversitySchool of Biology and Basic Medical Sciences, Soochow UniversitySchool of Biology and Basic Medical Sciences, Soochow UniversitySchool of Biology and Basic Medical Sciences, Soochow UniversitySchool of Biology and Basic Medical Sciences, Soochow UniversitySchool of Biology and Basic Medical Sciences, Soochow UniversitySchool of Biology and Basic Medical Sciences, Soochow UniversitySchool of Biology and Basic Medical Sciences, Soochow UniversityAbstract Background The protective efficacy of avian influenza virus (AIV) vaccines is unsatisfactory due to the presence of various serotypes generated by genetic reassortment. Thus, immunization with a polyantigen chimeric epitope vaccine may be an effective strategy for protecting poultry from infection with different AIV subtypes. Methods Baculovirus has recently emerged as a novel and attractive gene delivery vehicle for animal cells. In the present study, a recombinant baculovirus BmNPV-CMV/THB-P10/CTLT containing a fused codon-optimized sequence (CTLT) of T lymphocyte epitopes from H1HA, H9HA, and H7HA AIV subtypes, and another fused codon-optimized sequence (THB) of Th and B cell epitopes from H1HA, H9HA, and H7HA AIV subtypes, driven by a baculovirus P10 promoter and cytomegalovirus CMV promoter, respectively, was constructed. Results Western blotting and cellular immunofluorescence demonstrated that the CTLT (THB) can be expressed in rBac-CMV/THB-P10/CTLT-infected silkworm cells (mammalian HEK293T cells). Furthermore, the recombinant virus, rBac-CMV-THB-CTLT, was used to immunize both chickens and mice. Conclusions The results of an indirect ELISA, immunohistochemistry, and T lymphocyte proliferation assay indicated that specific humoral and cellular responses were detected in both chicken and mice. These results suggest that rBac-CMV/THB-P10/CTLT can be developed as a potential vaccine against different AIV subtypes.http://link.springer.com/article/10.1186/s12985-020-01388-wavian influenza viruspolyantigen epitope vaccineimmune responsebaculoviruschicken and mice |
| spellingShingle | Lei Yu Jun Pan Guangli Cao Mengsheng Jiang Yunshan Zhang Min Zhu Zi Liang Xing Zhang Xiaolong Hu Renyu Xue Chengliang Gong AIV polyantigen epitope expressed by recombinant baculovirus induces a systemic immune response in chicken and mouse models Virology Journal avian influenza virus polyantigen epitope vaccine immune response baculovirus chicken and mice |
| title | AIV polyantigen epitope expressed by recombinant baculovirus induces a systemic immune response in chicken and mouse models |
| title_full | AIV polyantigen epitope expressed by recombinant baculovirus induces a systemic immune response in chicken and mouse models |
| title_fullStr | AIV polyantigen epitope expressed by recombinant baculovirus induces a systemic immune response in chicken and mouse models |
| title_full_unstemmed | AIV polyantigen epitope expressed by recombinant baculovirus induces a systemic immune response in chicken and mouse models |
| title_short | AIV polyantigen epitope expressed by recombinant baculovirus induces a systemic immune response in chicken and mouse models |
| title_sort | aiv polyantigen epitope expressed by recombinant baculovirus induces a systemic immune response in chicken and mouse models |
| topic | avian influenza virus polyantigen epitope vaccine immune response baculovirus chicken and mice |
| url | http://link.springer.com/article/10.1186/s12985-020-01388-w |
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