Isolation and characterization of novel peptides from fermented products of Lactobacillus for ulcerative colitis prevention and treatment
Ulcerative colitis (UC) is an incurable and highly complex digestive disease affecting millions of people worldwide. Compared to the current therapeutic drugs, bioactive peptides are more promising and safe substances as functional foods or drugs for the prevention and treatment of UC. The alcohol-s...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Tsinghua University Press
2022-11-01
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| Series: | Food Science and Human Wellness |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213453022000866 |
| _version_ | 1797704198048972800 |
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| author | Dong He Wen Zeng Yi Wang Yifan Xing Kang Xiong Nan Su Chong Zhang Yuan Lu Xinhui Xing |
| author_facet | Dong He Wen Zeng Yi Wang Yifan Xing Kang Xiong Nan Su Chong Zhang Yuan Lu Xinhui Xing |
| author_sort | Dong He |
| collection | DOAJ |
| description | Ulcerative colitis (UC) is an incurable and highly complex digestive disease affecting millions of people worldwide. Compared to the current therapeutic drugs, bioactive peptides are more promising and safe substances as functional foods or drugs for the prevention and treatment of UC. The alcohol-soluble components from fermentation broth by fresh wheat germ and apple (AC-WGAF) were found to be effective in UC prevention in dextran sulfate sodium-induced mice in vivo. Herein, 4 novel peptides are identified from AC-WGAF by membrane ultrafiltration, recycling preparative high-performance liquid chromatography, and matrix-assisted laser desorption–ionization time-of-flight/time-of-flight mass spectrometry, possessing anti-colitis activity via using an in vitro model. One of those peptides named T24 (PVLGPVRGPFPLL) exhibited the most remarkable anti-colitis activity by preventing tight junction protein loss, maintaining epithelial barrier integrity, and promoting cell proliferation during in vitro and in vivo studies by regulating mitogen-activated protein kinase signaling pathways. Thus, T24 is a promising peptide as a functional food or novel drug for UC prevention and treatment. |
| first_indexed | 2024-03-12T05:16:46Z |
| format | Article |
| id | doaj.art-86363fd22f0d478db588582fef0c28dc |
| institution | Directory Open Access Journal |
| issn | 2213-4530 |
| language | English |
| last_indexed | 2024-03-12T05:16:46Z |
| publishDate | 2022-11-01 |
| publisher | Tsinghua University Press |
| record_format | Article |
| series | Food Science and Human Wellness |
| spelling | doaj.art-86363fd22f0d478db588582fef0c28dc2023-09-03T08:03:01ZengTsinghua University PressFood Science and Human Wellness2213-45302022-11-0111614641474Isolation and characterization of novel peptides from fermented products of Lactobacillus for ulcerative colitis prevention and treatmentDong He0Wen Zeng1Yi Wang2Yifan Xing3Kang Xiong4Nan Su5Chong Zhang6Yuan Lu7Xinhui Xing8School of Chemical Engineering and Light Industry, Guangdong University of Technology, Guangzhou 510006, China; Key Laboratory for Industrial Biocatalysis, Ministry of Education, Institute of Biochemical Engineering, Department of Chemical Engineering, China Center for Synthetic and Systems Biology, Tsinghua University, Beijing 100084, ChinaKey Laboratory for Industrial Biocatalysis, Ministry of Education, Institute of Biochemical Engineering, Department of Chemical Engineering, China Center for Synthetic and Systems Biology, Tsinghua University, Beijing 100084, ChinaKey Laboratory for Industrial Biocatalysis, Ministry of Education, Institute of Biochemical Engineering, Department of Chemical Engineering, China Center for Synthetic and Systems Biology, Tsinghua University, Beijing 100084, ChinaKey Laboratory for Industrial Biocatalysis, Ministry of Education, Institute of Biochemical Engineering, Department of Chemical Engineering, China Center for Synthetic and Systems Biology, Tsinghua University, Beijing 100084, ChinaKey Laboratory for Industrial Biocatalysis, Ministry of Education, Institute of Biochemical Engineering, Department of Chemical Engineering, China Center for Synthetic and Systems Biology, Tsinghua University, Beijing 100084, ChinaKey Laboratory for Industrial Biocatalysis, Ministry of Education, Institute of Biochemical Engineering, Department of Chemical Engineering, China Center for Synthetic and Systems Biology, Tsinghua University, Beijing 100084, ChinaKey Laboratory for Industrial Biocatalysis, Ministry of Education, Institute of Biochemical Engineering, Department of Chemical Engineering, China Center for Synthetic and Systems Biology, Tsinghua University, Beijing 100084, China; Center for Synthetic and Systems Biology, Tsinghua University, Beijing 100084, ChinaKey Laboratory for Industrial Biocatalysis, Ministry of Education, Institute of Biochemical Engineering, Department of Chemical Engineering, China Center for Synthetic and Systems Biology, Tsinghua University, Beijing 100084, ChinaKey Laboratory for Industrial Biocatalysis, Ministry of Education, Institute of Biochemical Engineering, Department of Chemical Engineering, China Center for Synthetic and Systems Biology, Tsinghua University, Beijing 100084, China; Center for Synthetic and Systems Biology, Tsinghua University, Beijing 100084, China; Institute of Biopharmaceutical and Health Engineering, Tsinghua Shenzhen International Graduate School, Shenzhen 518055, China; Institute of Biomedical Health Technology and Engineering, Shenzhen Bay Laboratory, Shenzhen 440300, China; Corresponding authors at: Tsinghua University, Beijing 100084, China.Ulcerative colitis (UC) is an incurable and highly complex digestive disease affecting millions of people worldwide. Compared to the current therapeutic drugs, bioactive peptides are more promising and safe substances as functional foods or drugs for the prevention and treatment of UC. The alcohol-soluble components from fermentation broth by fresh wheat germ and apple (AC-WGAF) were found to be effective in UC prevention in dextran sulfate sodium-induced mice in vivo. Herein, 4 novel peptides are identified from AC-WGAF by membrane ultrafiltration, recycling preparative high-performance liquid chromatography, and matrix-assisted laser desorption–ionization time-of-flight/time-of-flight mass spectrometry, possessing anti-colitis activity via using an in vitro model. One of those peptides named T24 (PVLGPVRGPFPLL) exhibited the most remarkable anti-colitis activity by preventing tight junction protein loss, maintaining epithelial barrier integrity, and promoting cell proliferation during in vitro and in vivo studies by regulating mitogen-activated protein kinase signaling pathways. Thus, T24 is a promising peptide as a functional food or novel drug for UC prevention and treatment.http://www.sciencedirect.com/science/article/pii/S2213453022000866Ulcerative colitisPeptideFermentation productIntestinal epithelial barrierCell proliferationMitogen-activated protein kinase signaling pathways |
| spellingShingle | Dong He Wen Zeng Yi Wang Yifan Xing Kang Xiong Nan Su Chong Zhang Yuan Lu Xinhui Xing Isolation and characterization of novel peptides from fermented products of Lactobacillus for ulcerative colitis prevention and treatment Food Science and Human Wellness Ulcerative colitis Peptide Fermentation product Intestinal epithelial barrier Cell proliferation Mitogen-activated protein kinase signaling pathways |
| title | Isolation and characterization of novel peptides from fermented products of Lactobacillus for ulcerative colitis prevention and treatment |
| title_full | Isolation and characterization of novel peptides from fermented products of Lactobacillus for ulcerative colitis prevention and treatment |
| title_fullStr | Isolation and characterization of novel peptides from fermented products of Lactobacillus for ulcerative colitis prevention and treatment |
| title_full_unstemmed | Isolation and characterization of novel peptides from fermented products of Lactobacillus for ulcerative colitis prevention and treatment |
| title_short | Isolation and characterization of novel peptides from fermented products of Lactobacillus for ulcerative colitis prevention and treatment |
| title_sort | isolation and characterization of novel peptides from fermented products of lactobacillus for ulcerative colitis prevention and treatment |
| topic | Ulcerative colitis Peptide Fermentation product Intestinal epithelial barrier Cell proliferation Mitogen-activated protein kinase signaling pathways |
| url | http://www.sciencedirect.com/science/article/pii/S2213453022000866 |
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