Antidiabetic and Hypolipidemic Efficiency of <i>Lactobacillus plantarum</i> Fermented Oat (<i>Avena sativa</i>) Extract in Streptozotocin-Induced Diabetes in Rats
Antidiabetic properties of fermented foods have been previously demonstrated. This study aimed to examine the antidiabetic and hypolipidemic potential activities of <i>L. plantarum</i> fermented oat extract in Streptozotocin-induced diabetic rats. Firstly, inoculating 1% of <i>L. p...
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MDPI AG
2022-06-01
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| Series: | Fermentation |
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| Online Access: | https://www.mdpi.com/2311-5637/8/6/267 |
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| author | Raya Algonaiman Hend F. Alharbi Hassan Barakat |
| author_facet | Raya Algonaiman Hend F. Alharbi Hassan Barakat |
| author_sort | Raya Algonaiman |
| collection | DOAJ |
| description | Antidiabetic properties of fermented foods have been previously demonstrated. This study aimed to examine the antidiabetic and hypolipidemic potential activities of <i>L. plantarum</i> fermented oat extract in Streptozotocin-induced diabetic rats. Firstly, inoculating 1% of <i>L. plantarum</i> starter culture in 10% whole oat flour in aqueous media resulted in 8.36 log CFU mL<sup>−1</sup> and pH 4.60 after 72 h of fermentation at 37 °C. With time progression of oat fermentation, total phenolic content (TPC), antioxidant activity (AOA), and γ-aminobutyric acid (GABA) contents were significantly increased up to 72 h. On the contrary, a significant reduction in β-glucan content was observed only after 72 h of fermentation. Secondly, separated aqueous extracts, i.e., unfermented oat extract (UFOE) and <i>L. plantarum</i> fermented oat extract (LFOE) were examined in vivo in a rat model, which consisted of five groups. Group 1 (negative group, NR); GROUP 2 (positive group, STZ), intraperitoneally injected with a single dose of 45 mg kg<sup>−1</sup> BW of Streptozotocin and administered 7 mL of distilled water orally per day; Group 3 (STZ+MET), diabetic rats orally administered 50 mg of metformin kg<sup>−1</sup> BW daily; Group 4 (STZ+UFOE), diabetic rats orally administered 7 mL of UFOE daily; and Group 5 (STZ+UFOE), diabetic rats orally administered 7 mL of LFOE daily for 6 weeks. Monitoring random blood glucose (RBG) and fasting blood glucose (FBG) showed that both the UFOE and the LFOE alleviated hyperglycemia in the STZ-induced diabetic rats. The extracts were significantly efficient in improving serum lipid profiles as compared with the positive group. Moreover, liver and kidneys’ functions were improved, and both extracts promoted hepatoprotective and nephroprotective characteristics. Furthermore, the administration of the UFOE and the LFOE efficiently attenuated GSH, CAT, and SOD enzymes and decreased MDA levels as compared with the positive group. In conclusion, data indicate the potential of UFOE and LFOE in future strategies as functional supplements against diabetes and diabetes-related complications. |
| first_indexed | 2024-03-09T23:50:47Z |
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| institution | Directory Open Access Journal |
| issn | 2311-5637 |
| language | English |
| last_indexed | 2024-03-09T23:50:47Z |
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| spelling | doaj.art-8677c2954fe34f29bde97faf54b2c32f2023-11-23T16:34:55ZengMDPI AGFermentation2311-56372022-06-018626710.3390/fermentation8060267Antidiabetic and Hypolipidemic Efficiency of <i>Lactobacillus plantarum</i> Fermented Oat (<i>Avena sativa</i>) Extract in Streptozotocin-Induced Diabetes in RatsRaya Algonaiman0Hend F. Alharbi1Hassan Barakat2Department of Food Science and Human Nutrition, College of Agriculture and Veterinary Medicine, Qassim University, Buraydah 51452, Saudi ArabiaDepartment of Food Science and Human Nutrition, College of Agriculture and Veterinary Medicine, Qassim University, Buraydah 51452, Saudi ArabiaDepartment of Food Science and Human Nutrition, College of Agriculture and Veterinary Medicine, Qassim University, Buraydah 51452, Saudi ArabiaAntidiabetic properties of fermented foods have been previously demonstrated. This study aimed to examine the antidiabetic and hypolipidemic potential activities of <i>L. plantarum</i> fermented oat extract in Streptozotocin-induced diabetic rats. Firstly, inoculating 1% of <i>L. plantarum</i> starter culture in 10% whole oat flour in aqueous media resulted in 8.36 log CFU mL<sup>−1</sup> and pH 4.60 after 72 h of fermentation at 37 °C. With time progression of oat fermentation, total phenolic content (TPC), antioxidant activity (AOA), and γ-aminobutyric acid (GABA) contents were significantly increased up to 72 h. On the contrary, a significant reduction in β-glucan content was observed only after 72 h of fermentation. Secondly, separated aqueous extracts, i.e., unfermented oat extract (UFOE) and <i>L. plantarum</i> fermented oat extract (LFOE) were examined in vivo in a rat model, which consisted of five groups. Group 1 (negative group, NR); GROUP 2 (positive group, STZ), intraperitoneally injected with a single dose of 45 mg kg<sup>−1</sup> BW of Streptozotocin and administered 7 mL of distilled water orally per day; Group 3 (STZ+MET), diabetic rats orally administered 50 mg of metformin kg<sup>−1</sup> BW daily; Group 4 (STZ+UFOE), diabetic rats orally administered 7 mL of UFOE daily; and Group 5 (STZ+UFOE), diabetic rats orally administered 7 mL of LFOE daily for 6 weeks. Monitoring random blood glucose (RBG) and fasting blood glucose (FBG) showed that both the UFOE and the LFOE alleviated hyperglycemia in the STZ-induced diabetic rats. The extracts were significantly efficient in improving serum lipid profiles as compared with the positive group. Moreover, liver and kidneys’ functions were improved, and both extracts promoted hepatoprotective and nephroprotective characteristics. Furthermore, the administration of the UFOE and the LFOE efficiently attenuated GSH, CAT, and SOD enzymes and decreased MDA levels as compared with the positive group. In conclusion, data indicate the potential of UFOE and LFOE in future strategies as functional supplements against diabetes and diabetes-related complications.https://www.mdpi.com/2311-5637/8/6/267<i>Lactobacillus plantarum</i>fermented oathypoglycemiahypolipidemiadiabetes |
| spellingShingle | Raya Algonaiman Hend F. Alharbi Hassan Barakat Antidiabetic and Hypolipidemic Efficiency of <i>Lactobacillus plantarum</i> Fermented Oat (<i>Avena sativa</i>) Extract in Streptozotocin-Induced Diabetes in Rats Fermentation <i>Lactobacillus plantarum</i> fermented oat hypoglycemia hypolipidemia diabetes |
| title | Antidiabetic and Hypolipidemic Efficiency of <i>Lactobacillus plantarum</i> Fermented Oat (<i>Avena sativa</i>) Extract in Streptozotocin-Induced Diabetes in Rats |
| title_full | Antidiabetic and Hypolipidemic Efficiency of <i>Lactobacillus plantarum</i> Fermented Oat (<i>Avena sativa</i>) Extract in Streptozotocin-Induced Diabetes in Rats |
| title_fullStr | Antidiabetic and Hypolipidemic Efficiency of <i>Lactobacillus plantarum</i> Fermented Oat (<i>Avena sativa</i>) Extract in Streptozotocin-Induced Diabetes in Rats |
| title_full_unstemmed | Antidiabetic and Hypolipidemic Efficiency of <i>Lactobacillus plantarum</i> Fermented Oat (<i>Avena sativa</i>) Extract in Streptozotocin-Induced Diabetes in Rats |
| title_short | Antidiabetic and Hypolipidemic Efficiency of <i>Lactobacillus plantarum</i> Fermented Oat (<i>Avena sativa</i>) Extract in Streptozotocin-Induced Diabetes in Rats |
| title_sort | antidiabetic and hypolipidemic efficiency of i lactobacillus plantarum i fermented oat i avena sativa i extract in streptozotocin induced diabetes in rats |
| topic | <i>Lactobacillus plantarum</i> fermented oat hypoglycemia hypolipidemia diabetes |
| url | https://www.mdpi.com/2311-5637/8/6/267 |
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