Directed differentiation of human pluripotent stem cells to epicardial-derived fibroblasts

Summary: Cardiac fibroblasts (CFBs) are a key therapeutic target due to their supportive roles during heart development and response to injury and disease. Here, we describe a robust protocol to differentiate human pluripotent stem cells (hPSCs) into CFBs through an epicardial intermediate. We discu...

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Bibliographic Details
Main Authors: Martha E. Floy, Fathima Shabnam, Sean P. Palecek
Format: Article
Language:English
Published: Elsevier 2022-06-01
Series:STAR Protocols
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166722001551