Metabolome and transcriptome profiling revealed the enhanced synthesis of volatile esters in Korla pear
Abstract Background Flavor contributes to the sensory quality of fruits, including taste and aroma aspects. The quality of foods is related to their flavor-associated compounds. Pear fruits have a fruity sense of smell, and esters are the main contributor of the aroma. Korla pear are well known due...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2023-05-01
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| Series: | BMC Plant Biology |
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| Online Access: | https://doi.org/10.1186/s12870-023-04264-1 |
| _version_ | 1827943532593152000 |
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| author | Yuan Liu Huan Wen Xiaoping Yang Cuiyun Wu Jiaqi Ming Hongyan Zhang Jiajing Chen Jiangbo Wang Juan Xu |
| author_facet | Yuan Liu Huan Wen Xiaoping Yang Cuiyun Wu Jiaqi Ming Hongyan Zhang Jiajing Chen Jiangbo Wang Juan Xu |
| author_sort | Yuan Liu |
| collection | DOAJ |
| description | Abstract Background Flavor contributes to the sensory quality of fruits, including taste and aroma aspects. The quality of foods is related to their flavor-associated compounds. Pear fruits have a fruity sense of smell, and esters are the main contributor of the aroma. Korla pear are well known due to its unique aroma, but the mechanism and genes related to volatile synthesis have not been fully investigated. Results Flavor-associated compounds, including 18 primary metabolites and 144 volatiles, were characterized in maturity fruits of ten pear cultivars from five species, respectively. Based on the varied metabolites profiles, the cultivars could be grouped into species, respectively, by using orthogonal partial least squares discrimination analysis (OPLS-DA). Simultaneously, 14 volatiles were selected as biomarkers to discriminate Korla pear (Pyrus sinkiangensis) from others. Correlation network analysis further revealed the biosynthetic pathways of the compounds in pear cultivars. Furthermore, the volatile profile in Korla pear throughout fruit development was investigated. Aldehydes were the most abundant volatiles, while numerous esters consistently accumulated especially at the maturity stages. Combined with transcriptomic and metabolic analysis, Ps5LOXL, PsADHL, and PsAATL were screened out as the key genes in ester synthesis. Conclusion Pear species can be distinguished by their metabolic profiles. The most diversified volatiles as well as esters was found in Korla pear, in which the enhancement of lipoxygenase pathway may lead to the high level of volatile esters at maturity stages. The study will benefit the fully usage of pear germplasm resources to serve fruit flavor breeding goals. |
| first_indexed | 2024-03-13T10:16:51Z |
| format | Article |
| id | doaj.art-86ad9100135c4ff883fc5327b91831e6 |
| institution | Directory Open Access Journal |
| issn | 1471-2229 |
| language | English |
| last_indexed | 2024-03-13T10:16:51Z |
| publishDate | 2023-05-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Plant Biology |
| spelling | doaj.art-86ad9100135c4ff883fc5327b91831e62023-05-21T11:12:37ZengBMCBMC Plant Biology1471-22292023-05-0123111110.1186/s12870-023-04264-1Metabolome and transcriptome profiling revealed the enhanced synthesis of volatile esters in Korla pearYuan Liu0Huan Wen1Xiaoping Yang2Cuiyun Wu3Jiaqi Ming4Hongyan Zhang5Jiajing Chen6Jiangbo Wang7Juan Xu8National Key Laboratory for Germplasm Innovation & Utilization of Horticultural Crops, College of Horticulture and Forestry Sciences, Huazhong Agricultural UniversityNational Key Laboratory for Germplasm Innovation & Utilization of Horticultural Crops, College of Horticulture and Forestry Sciences, Huazhong Agricultural UniversityInstitute of Fruit and Tea, Hubei Academy of Agricultural SciencesThe National and Local Joint Engineering Laboratory of High Efficiency and Superior-Quality Cultivation and Fruit Deep Processing Technology of Characteristic Fruit Trees in Southern Xinjiang, College of Horticulture and Forestry, Tarim UniversityGanzhou Agricultural Technology Extension CenterNational Key Laboratory for Germplasm Innovation & Utilization of Horticultural Crops, College of Horticulture and Forestry Sciences, Huazhong Agricultural UniversityNational Key Laboratory for Germplasm Innovation & Utilization of Horticultural Crops, College of Horticulture and Forestry Sciences, Huazhong Agricultural UniversityThe National and Local Joint Engineering Laboratory of High Efficiency and Superior-Quality Cultivation and Fruit Deep Processing Technology of Characteristic Fruit Trees in Southern Xinjiang, College of Horticulture and Forestry, Tarim UniversityNational Key Laboratory for Germplasm Innovation & Utilization of Horticultural Crops, College of Horticulture and Forestry Sciences, Huazhong Agricultural UniversityAbstract Background Flavor contributes to the sensory quality of fruits, including taste and aroma aspects. The quality of foods is related to their flavor-associated compounds. Pear fruits have a fruity sense of smell, and esters are the main contributor of the aroma. Korla pear are well known due to its unique aroma, but the mechanism and genes related to volatile synthesis have not been fully investigated. Results Flavor-associated compounds, including 18 primary metabolites and 144 volatiles, were characterized in maturity fruits of ten pear cultivars from five species, respectively. Based on the varied metabolites profiles, the cultivars could be grouped into species, respectively, by using orthogonal partial least squares discrimination analysis (OPLS-DA). Simultaneously, 14 volatiles were selected as biomarkers to discriminate Korla pear (Pyrus sinkiangensis) from others. Correlation network analysis further revealed the biosynthetic pathways of the compounds in pear cultivars. Furthermore, the volatile profile in Korla pear throughout fruit development was investigated. Aldehydes were the most abundant volatiles, while numerous esters consistently accumulated especially at the maturity stages. Combined with transcriptomic and metabolic analysis, Ps5LOXL, PsADHL, and PsAATL were screened out as the key genes in ester synthesis. Conclusion Pear species can be distinguished by their metabolic profiles. The most diversified volatiles as well as esters was found in Korla pear, in which the enhancement of lipoxygenase pathway may lead to the high level of volatile esters at maturity stages. The study will benefit the fully usage of pear germplasm resources to serve fruit flavor breeding goals.https://doi.org/10.1186/s12870-023-04264-1‘Korla pear’ (Pyrus sinkiangensis)FlavorPrimary metabolitesVolatilesEsters biosynthetic genesOPLS-DA |
| spellingShingle | Yuan Liu Huan Wen Xiaoping Yang Cuiyun Wu Jiaqi Ming Hongyan Zhang Jiajing Chen Jiangbo Wang Juan Xu Metabolome and transcriptome profiling revealed the enhanced synthesis of volatile esters in Korla pear BMC Plant Biology ‘Korla pear’ (Pyrus sinkiangensis) Flavor Primary metabolites Volatiles Esters biosynthetic genes OPLS-DA |
| title | Metabolome and transcriptome profiling revealed the enhanced synthesis of volatile esters in Korla pear |
| title_full | Metabolome and transcriptome profiling revealed the enhanced synthesis of volatile esters in Korla pear |
| title_fullStr | Metabolome and transcriptome profiling revealed the enhanced synthesis of volatile esters in Korla pear |
| title_full_unstemmed | Metabolome and transcriptome profiling revealed the enhanced synthesis of volatile esters in Korla pear |
| title_short | Metabolome and transcriptome profiling revealed the enhanced synthesis of volatile esters in Korla pear |
| title_sort | metabolome and transcriptome profiling revealed the enhanced synthesis of volatile esters in korla pear |
| topic | ‘Korla pear’ (Pyrus sinkiangensis) Flavor Primary metabolites Volatiles Esters biosynthetic genes OPLS-DA |
| url | https://doi.org/10.1186/s12870-023-04264-1 |
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