Accelerated genome engineering of Pseudomonas putida by I‐SceI―mediated recombination and CRISPR‐Cas9 counterselection

Summary Pseudomonas species have become reliable platforms for bioproduction due to their capability to tolerate harsh conditions imposed by large‐scale bioprocesses and their remarkable resistance to diverse physicochemical stresses. The last few years have brought forth a variety of synthetic biol...

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Bibliographic Details
Main Authors: Nicolas T. Wirth, Ekaterina Kozaeva, Pablo I. Nikel
Format: Article
Language:English
Published: Wiley 2020-01-01
Series:Microbial Biotechnology
Online Access:https://doi.org/10.1111/1751-7915.13396