Combined DiI and Antibody Labeling Reveals Complex Dysgenesis of Hippocampal Dendritic Spines in a Mouse Model of Fragile X Syndrome

Combined DiI and Antibody Labeling Reveals Complex Dysgenesis of Hippocampal Dendritic Spines in a Mouse Model of Fragile X Syndrome

Structural, functional, and molecular alterations in excitatory spines are a common hallmark of many neurodevelopmental disorders including intellectual disability and autism. Here, we describe an optimized methodology, based on combined use of DiI and immunofluorescence, for rapid and sensitive cha...

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Bibliographic Details
Main Authors: Luisa Speranza, Kardelen Dalım Filiz, Sarah Goebel, Carla Perrone-Capano, Salvatore Pulcrano, Floriana Volpicelli, Anna Francesconi
Format: Article
Language:English
Published: MDPI AG 2022-10-01
Series:Biomedicines
Subjects:
DiIC<sub>18</sub>
dendritic spines
excitatory synapses
synaptopodin
Fragile X Syndrome
<i>Fmr1</i> knockout mouse
Online Access:https://www.mdpi.com/2227-9059/10/11/2692

Internet

https://www.mdpi.com/2227-9059/10/11/2692

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