Efficient CRISPR Mutagenesis in Sturgeon Demonstrates Its Utility in Large, Slow-Maturing Vertebrates

Efficient CRISPR Mutagenesis in Sturgeon Demonstrates Its Utility in Large, Slow-Maturing Vertebrates

In the last decade, the CRISPR/Cas9 bacterial virus defense system has been adapted as a user-friendly, efficient, and precise method for targeted mutagenesis in eukaryotes. Though CRISPR/Cas9 has proven effective in a diverse range of organisms, it is still most often used to create mutant lines in...

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Bibliographic Details
Main Authors: Jan Stundl, Vladimír Soukup, Roman Franěk, Anna Pospisilova, Viktorie Psutkova, Martin Pšenička, Robert Cerny, Marianne E. Bronner, Daniel Meulemans Medeiros, David Jandzik
Format: Article
Language:English
Published: Frontiers Media S.A. 2022-02-01
Series:Frontiers in Cell and Developmental Biology
Subjects:
CRISPR/Cas9
targeted mutagenesis
non-teleost fish
sturgeon
vertebrates
development
Online Access:https://www.frontiersin.org/articles/10.3389/fcell.2022.750833/full

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https://www.frontiersin.org/articles/10.3389/fcell.2022.750833/full

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