Determination of Transgene Copy Number and Expression Level Using Denaturing Gradient Gel Electrophoresis
Transgenic mice and cell lines are frequently developed to study human disease. Accurate determination of transgene copy number and levels of mRNA are necessary to understand the phenotypic changes observed in these models. Currently, transgene copy number and expression are estimated by Southern bl...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
1998-01-01
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| Series: | BioTechniques |
| Online Access: | https://www.future-science.com/doi/10.2144/98241st06 |