Coffee-Derived Phenolic Compounds Activate Nrf2 Antioxidant Pathway in I/R Injury In Vitro Model: A Nutritional Approach Preventing Age Related-Damages
Age-related injuries are often connected to alterations in redox homeostasis. The imbalance between free radical oxygen species and endogenous antioxidants defenses could be associated with a growing risk of transient ischemic attack and stroke. In this context, a daily supply of dietary antioxidant...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-02-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/27/3/1049 |
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| author | Elena Lonati Tatiana Carrozzini Ilaria Bruni Pedro Mena Laura Botto Emanuela Cazzaniga Daniele Del Rio Massimo Labra Paola Palestini Alessandra Bulbarelli |
| author_facet | Elena Lonati Tatiana Carrozzini Ilaria Bruni Pedro Mena Laura Botto Emanuela Cazzaniga Daniele Del Rio Massimo Labra Paola Palestini Alessandra Bulbarelli |
| author_sort | Elena Lonati |
| collection | DOAJ |
| description | Age-related injuries are often connected to alterations in redox homeostasis. The imbalance between free radical oxygen species and endogenous antioxidants defenses could be associated with a growing risk of transient ischemic attack and stroke. In this context, a daily supply of dietary antioxidants could counteract oxidative stress occurring during ischemia/reperfusion injury (I/R), preventing brain damage. Here we investigated the potential antioxidant properties of coffee-derived circulating metabolites and a coffee pulp phytoextract, testing their efficacy as ROS scavengers in an in vitro model of ischemia. Indeed, the coffee fruit is an important source of phenolic compounds, such as chlorogenic acids, present both in the brewed seed and in the discarded pulp. Therefore, rat brain endothelial cells, subjected to oxygen and glucose deprivation (OGD) and recovery (ogR) to mimic reperfusion, were pretreated or not with coffee by-products. The results indicate that, under OGD/ogR, the ROS accumulation was reduced by coffee by-product. Additionally, the coffee extract activated the Nrf2 antioxidant pathway via Erk and Akt kinases phosphorylation, as shown by increased Nrf2 and HO-1 protein levels. The data indicate that the daily intake of coffee by-products as a dietary food supplement represents a potential nutritional strategy to counteract aging. |
| first_indexed | 2024-03-09T23:26:30Z |
| format | Article |
| id | doaj.art-8709e20110fa42259891df062f068f8f |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-09T23:26:30Z |
| publishDate | 2022-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-8709e20110fa42259891df062f068f8f2023-11-23T17:16:47ZengMDPI AGMolecules1420-30492022-02-01273104910.3390/molecules27031049Coffee-Derived Phenolic Compounds Activate Nrf2 Antioxidant Pathway in I/R Injury In Vitro Model: A Nutritional Approach Preventing Age Related-DamagesElena Lonati0Tatiana Carrozzini1Ilaria Bruni2Pedro Mena3Laura Botto4Emanuela Cazzaniga5Daniele Del Rio6Massimo Labra7Paola Palestini8Alessandra Bulbarelli9School of Medicine and Surgery, University of Milano-Bicocca, 20900 Monza, ItalySchool of Medicine and Surgery, University of Milano-Bicocca, 20900 Monza, ItalyBicocca Center of Science and Technology for Food, University of Milano-Bicocca, 20126 Milano, ItalyHuman Nutrition Unit, Department of Food and Drug, University of Parma, 43124 Parma, ItalySchool of Medicine and Surgery, University of Milano-Bicocca, 20900 Monza, ItalySchool of Medicine and Surgery, University of Milano-Bicocca, 20900 Monza, ItalyHuman Nutrition Unit, Department of Food and Drug, University of Parma, 43124 Parma, ItalyBicocca Center of Science and Technology for Food, University of Milano-Bicocca, 20126 Milano, ItalySchool of Medicine and Surgery, University of Milano-Bicocca, 20900 Monza, ItalySchool of Medicine and Surgery, University of Milano-Bicocca, 20900 Monza, ItalyAge-related injuries are often connected to alterations in redox homeostasis. The imbalance between free radical oxygen species and endogenous antioxidants defenses could be associated with a growing risk of transient ischemic attack and stroke. In this context, a daily supply of dietary antioxidants could counteract oxidative stress occurring during ischemia/reperfusion injury (I/R), preventing brain damage. Here we investigated the potential antioxidant properties of coffee-derived circulating metabolites and a coffee pulp phytoextract, testing their efficacy as ROS scavengers in an in vitro model of ischemia. Indeed, the coffee fruit is an important source of phenolic compounds, such as chlorogenic acids, present both in the brewed seed and in the discarded pulp. Therefore, rat brain endothelial cells, subjected to oxygen and glucose deprivation (OGD) and recovery (ogR) to mimic reperfusion, were pretreated or not with coffee by-products. The results indicate that, under OGD/ogR, the ROS accumulation was reduced by coffee by-product. Additionally, the coffee extract activated the Nrf2 antioxidant pathway via Erk and Akt kinases phosphorylation, as shown by increased Nrf2 and HO-1 protein levels. The data indicate that the daily intake of coffee by-products as a dietary food supplement represents a potential nutritional strategy to counteract aging.https://www.mdpi.com/1420-3049/27/3/1049coffee phytoextractcoffee metabolitesphenolic compoundsoxidative stressNrf2/ARE antioxidant pathwayoxygen and glucose deprivation |
| spellingShingle | Elena Lonati Tatiana Carrozzini Ilaria Bruni Pedro Mena Laura Botto Emanuela Cazzaniga Daniele Del Rio Massimo Labra Paola Palestini Alessandra Bulbarelli Coffee-Derived Phenolic Compounds Activate Nrf2 Antioxidant Pathway in I/R Injury In Vitro Model: A Nutritional Approach Preventing Age Related-Damages Molecules coffee phytoextract coffee metabolites phenolic compounds oxidative stress Nrf2/ARE antioxidant pathway oxygen and glucose deprivation |
| title | Coffee-Derived Phenolic Compounds Activate Nrf2 Antioxidant Pathway in I/R Injury In Vitro Model: A Nutritional Approach Preventing Age Related-Damages |
| title_full | Coffee-Derived Phenolic Compounds Activate Nrf2 Antioxidant Pathway in I/R Injury In Vitro Model: A Nutritional Approach Preventing Age Related-Damages |
| title_fullStr | Coffee-Derived Phenolic Compounds Activate Nrf2 Antioxidant Pathway in I/R Injury In Vitro Model: A Nutritional Approach Preventing Age Related-Damages |
| title_full_unstemmed | Coffee-Derived Phenolic Compounds Activate Nrf2 Antioxidant Pathway in I/R Injury In Vitro Model: A Nutritional Approach Preventing Age Related-Damages |
| title_short | Coffee-Derived Phenolic Compounds Activate Nrf2 Antioxidant Pathway in I/R Injury In Vitro Model: A Nutritional Approach Preventing Age Related-Damages |
| title_sort | coffee derived phenolic compounds activate nrf2 antioxidant pathway in i r injury in vitro model a nutritional approach preventing age related damages |
| topic | coffee phytoextract coffee metabolites phenolic compounds oxidative stress Nrf2/ARE antioxidant pathway oxygen and glucose deprivation |
| url | https://www.mdpi.com/1420-3049/27/3/1049 |
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