Detection and Evaluation of Non-Recombinants in cDNA Libraries by Multiple Cloning Region PCR
Bacteriophages that are routinely used in cDNA libraries do not require any biological selection for forming plaques. Thus parental non-recombinant phages are always found in variable proportions together with recombinant ones in all cDNA libraries. The presence of non-recombinants in significant pr...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2002-01-01
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| Series: | BioTechniques |
| Online Access: | https://www.future-science.com/doi/10.2144/02321st05 |