Novel in situ pretreatment method for significantly enhancing the signal in MALDI-TOF MS of formalin-fixed paraffin-embedded tissue sections.

Novel in situ pretreatment method for significantly enhancing the signal in MALDI-TOF MS of formalin-fixed paraffin-embedded tissue sections.

The application of matrix-assisted laser desorption/ionization (MALDI)-based mass spectrometry (MS) to the proteomic analysis of formalin-fixed paraffin-embedded (FFPE) tissue presents significant technical challenges. In situ enzymatic digestion is frequently used to unlock formalin-fixed tissues f...

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Bibliographic Details
Main Authors: Yu Kakimoto, Tatsuaki Tsuruyama, Takushi Yamamoto, Masaru Furuta, Hirokazu Kotani, Munetaka Ozeki, Akihiko Yoshizawa, Hironori Haga, Keiji Tamaki
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2012-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC3416821?pdf=render

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http://europepmc.org/articles/PMC3416821?pdf=render

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