Pathological α-synuclein detected by real-time quaking-induced conversion in synucleinopathies
synucleinopathies are diseases characterized by the aggregation of α-synuclein (α-syn), which forms fibrils through misfolding and accumulates in a prion-like manner. To detect the presence of these α-syn aggregates in clinical samples, seed amplification assays (SAAs) have been developed. These SAA...
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Elsevier
2024-03-01
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| Series: | Experimental Gerontology |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0531556524000081 |
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| author | Juan Huang Xingxing Yuan Lin Chen Binbin Hu Hui Wang Ye Wang Wei Huang |
| author_facet | Juan Huang Xingxing Yuan Lin Chen Binbin Hu Hui Wang Ye Wang Wei Huang |
| author_sort | Juan Huang |
| collection | DOAJ |
| description | synucleinopathies are diseases characterized by the aggregation of α-synuclein (α-syn), which forms fibrils through misfolding and accumulates in a prion-like manner. To detect the presence of these α-syn aggregates in clinical samples, seed amplification assays (SAAs) have been developed. These SAAs are capable of amplifying the α-syn seeds, allowing for their detection. αSyn-SAAs have been reported under the names ‘protein misfolding cyclic amplification’ (αSyn-PMCA) and ‘real-time quaking-induced conversion'α-Syn-RT-QuIC. The α-Syn RT-QuIC, in particular, has been adapted to amplify and detect α-syn aggregates in various biospecimens, including cerebrospinal fluid (CSF), skin, nasal brushing, serum and saliva. The α-syn RT-QuIC assay has demonstrated good sensitivity and specificity in detecting pathological α-syn, particularly in Parkinson's disease (PD) and dementia with Lewy bodies (DLB) cases, with an accuracy rate of up to 80 %. Additionally, differential diagnosis between DLB and PD, as well as PD and multiple system atrophy (MSA), can be achieved by utilizing certain kinetic thioflavin T (ThT) parameters and other parameters. Moreover, the positive detection of α-syn in the prodromal stage of synucleinopathies provides an opportunity for early intervention and management. In summary, the development of the α-syn RT-QuIC assay has greatly contributed to the field of synucleinopathies. Therefore, we review the development of α-syn RT-QuIC assay and describe in detail the recent advancements of α-syn RT-QuIC assay for detecting pathological α-syn in synucleinopathies. |
| first_indexed | 2024-03-07T21:54:25Z |
| format | Article |
| id | doaj.art-873b9d9fc51647a28a51c50358d16450 |
| institution | Directory Open Access Journal |
| issn | 1873-6815 |
| language | English |
| last_indexed | 2024-03-07T21:54:25Z |
| publishDate | 2024-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Experimental Gerontology |
| spelling | doaj.art-873b9d9fc51647a28a51c50358d164502024-02-25T04:34:43ZengElsevierExperimental Gerontology1873-68152024-03-01187112366Pathological α-synuclein detected by real-time quaking-induced conversion in synucleinopathiesJuan Huang0Xingxing Yuan1Lin Chen2Binbin Hu3Hui Wang4Ye Wang5Wei Huang6Department of Neurology, Second Affiliated Hospital of Nanchang University, Jiangxi Medical College, Nanchang University, ChinaDepartment of Anesthesiology, Hunan Provincial People's Hospital, The First Affiliated Hospital of Hunan Normal University, ChinaDepartment of Neurology, Second Affiliated Hospital of Nanchang University, Jiangxi Medical College, Nanchang University, ChinaDepartment of Neurology, Second Affiliated Hospital of Nanchang University, Jiangxi Medical College, Nanchang University, ChinaDepartment of Neurology, Second Affiliated Hospital of Nanchang University, Jiangxi Medical College, Nanchang University, ChinaDepartment of Neurology, Second Affiliated Hospital of Nanchang University, Jiangxi Medical College, Nanchang University, China; Corresponding author.Department of Neurology, Second Affiliated Hospital of Nanchang University, Jiangxi Medical College, Nanchang University, China; Corresponding author.synucleinopathies are diseases characterized by the aggregation of α-synuclein (α-syn), which forms fibrils through misfolding and accumulates in a prion-like manner. To detect the presence of these α-syn aggregates in clinical samples, seed amplification assays (SAAs) have been developed. These SAAs are capable of amplifying the α-syn seeds, allowing for their detection. αSyn-SAAs have been reported under the names ‘protein misfolding cyclic amplification’ (αSyn-PMCA) and ‘real-time quaking-induced conversion'α-Syn-RT-QuIC. The α-Syn RT-QuIC, in particular, has been adapted to amplify and detect α-syn aggregates in various biospecimens, including cerebrospinal fluid (CSF), skin, nasal brushing, serum and saliva. The α-syn RT-QuIC assay has demonstrated good sensitivity and specificity in detecting pathological α-syn, particularly in Parkinson's disease (PD) and dementia with Lewy bodies (DLB) cases, with an accuracy rate of up to 80 %. Additionally, differential diagnosis between DLB and PD, as well as PD and multiple system atrophy (MSA), can be achieved by utilizing certain kinetic thioflavin T (ThT) parameters and other parameters. Moreover, the positive detection of α-syn in the prodromal stage of synucleinopathies provides an opportunity for early intervention and management. In summary, the development of the α-syn RT-QuIC assay has greatly contributed to the field of synucleinopathies. Therefore, we review the development of α-syn RT-QuIC assay and describe in detail the recent advancements of α-syn RT-QuIC assay for detecting pathological α-syn in synucleinopathies.http://www.sciencedirect.com/science/article/pii/S0531556524000081Seed amplification assaysα-Syn RT-QuICSelf-propagationAggregated α-synucleinSynucleinopathies |
| spellingShingle | Juan Huang Xingxing Yuan Lin Chen Binbin Hu Hui Wang Ye Wang Wei Huang Pathological α-synuclein detected by real-time quaking-induced conversion in synucleinopathies Experimental Gerontology Seed amplification assays α-Syn RT-QuIC Self-propagation Aggregated α-synuclein Synucleinopathies |
| title | Pathological α-synuclein detected by real-time quaking-induced conversion in synucleinopathies |
| title_full | Pathological α-synuclein detected by real-time quaking-induced conversion in synucleinopathies |
| title_fullStr | Pathological α-synuclein detected by real-time quaking-induced conversion in synucleinopathies |
| title_full_unstemmed | Pathological α-synuclein detected by real-time quaking-induced conversion in synucleinopathies |
| title_short | Pathological α-synuclein detected by real-time quaking-induced conversion in synucleinopathies |
| title_sort | pathological α synuclein detected by real time quaking induced conversion in synucleinopathies |
| topic | Seed amplification assays α-Syn RT-QuIC Self-propagation Aggregated α-synuclein Synucleinopathies |
| url | http://www.sciencedirect.com/science/article/pii/S0531556524000081 |
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