Detecting and quantifying liquid–liquid phase separation in living cells by model-free calibrated half-bleaching

Detecting and quantifying liquid–liquid phase separation in living cells by model-free calibrated half-bleaching

It is currently debated how to reliably distinguish liquid–liquid phase separation (LLPS) from other mechanisms. Here the authors report model-free calibrated half-FRAP (MOCHA-FRAP) to probe the barrier at the condensate interface that is responsible for preferential internal mixing in LLPS.

Bibliographic Details
Main Authors: Fernando Muzzopappa, Johan Hummert, Michela Anfossi, Stanimir Asenov Tashev, Dirk-Peter Herten, Fabian Erdel
Format: Article
Language:English
Published: Nature Portfolio 2022-12-01
Series:Nature Communications
Online Access:https://doi.org/10.1038/s41467-022-35430-y

Internet

https://doi.org/10.1038/s41467-022-35430-y

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