Detecting and quantifying liquid–liquid phase separation in living cells by model-free calibrated half-bleaching
It is currently debated how to reliably distinguish liquid–liquid phase separation (LLPS) from other mechanisms. Here the authors report model-free calibrated half-FRAP (MOCHA-FRAP) to probe the barrier at the condensate interface that is responsible for preferential internal mixing in LLPS.
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2022-12-01
|
| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-022-35430-y |
| _version_ | 1811196640328941568 |
|---|---|
| author | Fernando Muzzopappa Johan Hummert Michela Anfossi Stanimir Asenov Tashev Dirk-Peter Herten Fabian Erdel |
| author_facet | Fernando Muzzopappa Johan Hummert Michela Anfossi Stanimir Asenov Tashev Dirk-Peter Herten Fabian Erdel |
| author_sort | Fernando Muzzopappa |
| collection | DOAJ |
| description | It is currently debated how to reliably distinguish liquid–liquid phase separation (LLPS) from other mechanisms. Here the authors report model-free calibrated half-FRAP (MOCHA-FRAP) to probe the barrier at the condensate interface that is responsible for preferential internal mixing in LLPS. |
| first_indexed | 2024-04-12T01:02:21Z |
| format | Article |
| id | doaj.art-874443a150d04973b28034e0cceabdb3 |
| institution | Directory Open Access Journal |
| issn | 2041-1723 |
| language | English |
| last_indexed | 2024-04-12T01:02:21Z |
| publishDate | 2022-12-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj.art-874443a150d04973b28034e0cceabdb32022-12-22T03:54:24ZengNature PortfolioNature Communications2041-17232022-12-0113111510.1038/s41467-022-35430-yDetecting and quantifying liquid–liquid phase separation in living cells by model-free calibrated half-bleachingFernando Muzzopappa0Johan Hummert1Michela Anfossi2Stanimir Asenov Tashev3Dirk-Peter Herten4Fabian Erdel5Molecular, Cellular and Developmental Biology Unit (MCD), Center for Integrative Biology (CBI), CNRS, UPSCollege of Medical and Dental Sciences & School of Chemistry, University of BirminghamMolecular, Cellular and Developmental Biology Unit (MCD), Center for Integrative Biology (CBI), CNRS, UPSCollege of Medical and Dental Sciences & School of Chemistry, University of BirminghamCollege of Medical and Dental Sciences & School of Chemistry, University of BirminghamMolecular, Cellular and Developmental Biology Unit (MCD), Center for Integrative Biology (CBI), CNRS, UPSIt is currently debated how to reliably distinguish liquid–liquid phase separation (LLPS) from other mechanisms. Here the authors report model-free calibrated half-FRAP (MOCHA-FRAP) to probe the barrier at the condensate interface that is responsible for preferential internal mixing in LLPS.https://doi.org/10.1038/s41467-022-35430-y |
| spellingShingle | Fernando Muzzopappa Johan Hummert Michela Anfossi Stanimir Asenov Tashev Dirk-Peter Herten Fabian Erdel Detecting and quantifying liquid–liquid phase separation in living cells by model-free calibrated half-bleaching Nature Communications |
| title | Detecting and quantifying liquid–liquid phase separation in living cells by model-free calibrated half-bleaching |
| title_full | Detecting and quantifying liquid–liquid phase separation in living cells by model-free calibrated half-bleaching |
| title_fullStr | Detecting and quantifying liquid–liquid phase separation in living cells by model-free calibrated half-bleaching |
| title_full_unstemmed | Detecting and quantifying liquid–liquid phase separation in living cells by model-free calibrated half-bleaching |
| title_short | Detecting and quantifying liquid–liquid phase separation in living cells by model-free calibrated half-bleaching |
| title_sort | detecting and quantifying liquid liquid phase separation in living cells by model free calibrated half bleaching |
| url | https://doi.org/10.1038/s41467-022-35430-y |
| work_keys_str_mv | AT fernandomuzzopappa detectingandquantifyingliquidliquidphaseseparationinlivingcellsbymodelfreecalibratedhalfbleaching AT johanhummert detectingandquantifyingliquidliquidphaseseparationinlivingcellsbymodelfreecalibratedhalfbleaching AT michelaanfossi detectingandquantifyingliquidliquidphaseseparationinlivingcellsbymodelfreecalibratedhalfbleaching AT stanimirasenovtashev detectingandquantifyingliquidliquidphaseseparationinlivingcellsbymodelfreecalibratedhalfbleaching AT dirkpeterherten detectingandquantifyingliquidliquidphaseseparationinlivingcellsbymodelfreecalibratedhalfbleaching AT fabianerdel detectingandquantifyingliquidliquidphaseseparationinlivingcellsbymodelfreecalibratedhalfbleaching |