Evaluation of activity of peroxidases contained in various plant sources
In this study, the decolorization reaction of aqueous solution bromophenol blue dye was carried out in the presence of hydrogen peroxide and peroxidase contained in the roots of horseradish (Armoracia rusticana), radish (Raphanus sativus var. radicula), yellow turnip (Brassica napobrassica), daikon...
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| Format: | Article |
| Language: | English |
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Saratov State University
2023-12-01
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| Series: | Известия Саратовского университета. Новая серия: Серия Химия. Биология. Экология |
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| Online Access: | https://ichbe.sgu.ru/sites/ichbe.sgu.ru/files/text-pdf/2023/12/himiya_2023_4-472-478.pdf |
| _version_ | 1827398894819999744 |
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| author | Solovyeva, Anna A. Lebedeva, Olga E. Thi Pham, Trinh |
| author_facet | Solovyeva, Anna A. Lebedeva, Olga E. Thi Pham, Trinh |
| author_sort | Solovyeva, Anna A. |
| collection | DOAJ |
| description | In this study, the decolorization reaction of aqueous solution bromophenol blue dye was carried out in the presence of hydrogen peroxide and peroxidase contained in the roots of horseradish (Armoracia rusticana), radish (Raphanus sativus var. radicula), yellow turnip (Brassica napobrassica), daikon (Raphanus sativus), black radish (Raphanus sativus ‘Niger’), green radish (Raphanus) or stalk of white cabbage (Brassica capitata). The reaction was carried out at an initial dye content of 25,1 μM, a hydrogen peroxide concentration of 0,4 mM, at t = 24 °C and pH 4,01, the peel of various vegetables was used as a source of peroxidase; it was inserted into the reaction without enzyme primary extraction. When using horseradish waste, black radish, white cabbage, turnips, it is possible to get the degree of decolorization of the dye in its aqueous solution by 95%. The maximum rate and Michaelis constants of the enzymatic reaction are calculated by the linearization method in the Linuiver – Burke coordinates. It is shown that the activity of peroxidase contained in the peel of black radish persists with an increase in temperature from 23 to 40 °С. |
| first_indexed | 2024-03-08T19:36:28Z |
| format | Article |
| id | doaj.art-8744c66a532241a1ad6f8a8b6e3dce2e |
| institution | Directory Open Access Journal |
| issn | 1816-9775 2541-8971 |
| language | English |
| last_indexed | 2024-03-08T19:36:28Z |
| publishDate | 2023-12-01 |
| publisher | Saratov State University |
| record_format | Article |
| series | Известия Саратовского университета. Новая серия: Серия Химия. Биология. Экология |
| spelling | doaj.art-8744c66a532241a1ad6f8a8b6e3dce2e2023-12-26T00:38:04ZengSaratov State UniversityИзвестия Саратовского университета. Новая серия: Серия Химия. Биология. Экология1816-97752541-89712023-12-0123447247810.18500/1816-9775-2023-23-4-472-478Evaluation of activity of peroxidases contained in various plant sourcesSolovyeva, Anna A.0Lebedeva, Olga E.1Thi Pham, Trinh2Federal State Autonomous Educational Institution of Higher Education, 85 Pobedy St., Belgorod 308015, RussiaFederal State Autonomous Educational Institution of Higher Education, 85 Pobedy St., Belgorod 308015, RussiaFederal State Autonomous Educational Institution of Higher Education, 85 Pobedy St., Belgorod 308015, RussiaIn this study, the decolorization reaction of aqueous solution bromophenol blue dye was carried out in the presence of hydrogen peroxide and peroxidase contained in the roots of horseradish (Armoracia rusticana), radish (Raphanus sativus var. radicula), yellow turnip (Brassica napobrassica), daikon (Raphanus sativus), black radish (Raphanus sativus ‘Niger’), green radish (Raphanus) or stalk of white cabbage (Brassica capitata). The reaction was carried out at an initial dye content of 25,1 μM, a hydrogen peroxide concentration of 0,4 mM, at t = 24 °C and pH 4,01, the peel of various vegetables was used as a source of peroxidase; it was inserted into the reaction without enzyme primary extraction. When using horseradish waste, black radish, white cabbage, turnips, it is possible to get the degree of decolorization of the dye in its aqueous solution by 95%. The maximum rate and Michaelis constants of the enzymatic reaction are calculated by the linearization method in the Linuiver – Burke coordinates. It is shown that the activity of peroxidase contained in the peel of black radish persists with an increase in temperature from 23 to 40 °С.https://ichbe.sgu.ru/sites/ichbe.sgu.ru/files/text-pdf/2023/12/himiya_2023_4-472-478.pdfhorseradish peroxidasebromophenol blue dyeenzymatic decolorization |
| spellingShingle | Solovyeva, Anna A. Lebedeva, Olga E. Thi Pham, Trinh Evaluation of activity of peroxidases contained in various plant sources Известия Саратовского университета. Новая серия: Серия Химия. Биология. Экология horseradish peroxidase bromophenol blue dye enzymatic decolorization |
| title | Evaluation of activity of peroxidases contained in various plant sources |
| title_full | Evaluation of activity of peroxidases contained in various plant sources |
| title_fullStr | Evaluation of activity of peroxidases contained in various plant sources |
| title_full_unstemmed | Evaluation of activity of peroxidases contained in various plant sources |
| title_short | Evaluation of activity of peroxidases contained in various plant sources |
| title_sort | evaluation of activity of peroxidases contained in various plant sources |
| topic | horseradish peroxidase bromophenol blue dye enzymatic decolorization |
| url | https://ichbe.sgu.ru/sites/ichbe.sgu.ru/files/text-pdf/2023/12/himiya_2023_4-472-478.pdf |
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