Engineered domain-inlaid Nme2Cas9 adenine base editors with increased on-target DNA editing and targeting scope
Abstract Background Nme2ABE8e has been constructed and characterized as a compact, accurate adenine base editor with a less restrictive dinucleotide protospacer-adjacent motif (PAM: N4CC) but low editing efficiency at challenging loci in human cells. Here, we engineered a subset of domain-inlaid Nme...
Main Authors: | , , , , , , , , , , , |
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Format: | Article |
Language: | English |
Published: |
BMC
2023-11-01
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Series: | BMC Biology |
Subjects: | |
Online Access: | https://doi.org/10.1186/s12915-023-01754-4 |