Engineered domain-inlaid Nme2Cas9 adenine base editors with increased on-target DNA editing and targeting scope

Engineered domain-inlaid Nme2Cas9 adenine base editors with increased on-target DNA editing and targeting scope

Abstract Background Nme2ABE8e has been constructed and characterized as a compact, accurate adenine base editor with a less restrictive dinucleotide protospacer-adjacent motif (PAM: N4CC) but low editing efficiency at challenging loci in human cells. Here, we engineered a subset of domain-inlaid Nme...

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Bibliographic Details
Main Authors: Ding Zhao, Xun Gao, Jiale Zhou, Jinze Li, Yuqiang Qian, Di Wang, Wenchao Niu, Tao Zhang, Mingyang Hu, Haoyang Xiong, Liangxue Lai, Zhanjun Li
Format: Article
Language:English
Published: BMC 2023-11-01
Series:BMC Biology
Subjects:
Adenine base editors
Inlaid domain
Nme2ABE8e-797
Nme2ABE8e-797Smu
Nme2ABE8e-797−C
Online Access:https://doi.org/10.1186/s12915-023-01754-4

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