Evaluation of trabecular meshwork-specific promoters in vitro and in vivo using scAAV2 vectors expressing C3 transferase
AIM: To evaluate the potential of two trabecular meshwork (TM)-specific promoters, Chitinase 3-like 1 (Ch3L1) and matrix gla protein (MGP), for improving specificity and safety in glaucoma gene therapy based on self-complementary AAV2 (scAAV2) vector technologies. METHODS: An scAAV2 vector with C3 t...
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| Format: | Article |
| Language: | English |
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Press of International Journal of Ophthalmology (IJO PRESS)
2023-08-01
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| Series: | International Journal of Ophthalmology |
| Subjects: | |
| Online Access: | http://ies.ijo.cn/en_publish/2023/8/20230803.pdf |
| _version_ | 1827893938926649344 |
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| author | Jun-Kai Tan Ying Xiao Guo Liu Long-Xiang Huang Wen-Hao Ma Yan Xia Xi-Zhen Wang Xian-Jun Zhu Su-Ping Cai Xiao-Bing Wu Yun Wang Xu-Yang Liu |
| author_facet | Jun-Kai Tan Ying Xiao Guo Liu Long-Xiang Huang Wen-Hao Ma Yan Xia Xi-Zhen Wang Xian-Jun Zhu Su-Ping Cai Xiao-Bing Wu Yun Wang Xu-Yang Liu |
| author_sort | Jun-Kai Tan |
| collection | DOAJ |
| description | AIM: To evaluate the potential of two trabecular meshwork (TM)-specific promoters, Chitinase 3-like 1 (Ch3L1) and matrix gla protein (MGP), for improving specificity and safety in glaucoma gene therapy based on self-complementary AAV2 (scAAV2) vector technologies. METHODS: An scAAV2 vector with C3 transferase (C3) as the reporter gene (scAAV2-C3) was selected. The scAAV2-C3 vectors were driven by Ch3L1 (scAAV2-Ch3L1-C3), MGP (scAAV2-MGP-C3), enhanced MGP (scAAV2-eMGP-C3) and cytomegalovirus (scAAV2-CMV-C3), respectively. The cultured primary human TM cells were treated with each vector at different multiplicities of infections. Changes in cell morphology were observed by phase contrast microscopy. Actin stress fibers and Rho GTPases/Rho-associated protein kinase pathway-related molecules were assessed by immunofluorescence staining, real-time quantitative polymerase chain reaction and Western blot. Each vector was injected intracamerally into the one eye of each rat at low and high doses respectively. In vivo green fluorescence was visualized by a Micron III Retinal Imaging Microscope. Intraocular pressure (IOP) was monitored using a rebound tonometer. Ocular responses were evaluated by slit-lamp microscopy. Ocular histopathology analysis was examined by hematoxylin and eosin staining. RESULTS: In TM cell culture studies, the vector-mediated C3 expression induced morphologic changes, disruption of actin cytoskeleton and reduction of fibronectin expression in TM cells by inhibiting the Rho GTPases/Rho-associated protein kinase signaling pathway. At the same dose, these changes were significant in TM cells treated with scAAV2-CMV-C3 or scAAV2-Ch3L1-C3, but not in cells treated with scAAV2-eMGP-C3 or scAAV2-MGP-C3. At low-injected dose, the IOP was significantly decreased in the scAAV2-Ch3L1-C3-injected eyes but not in scAAV2-MGP-C3-injected and scAAV2-eMGP-C3-injected eyes. At high-injected dose, significant IOP reduction was observed in the scAAV2-eMGP-C3-injected eyes but not in scAAV2-MGP-C3-injected eyes. Similar to scAAV2-CMV-C3, scAAV2-Ch3L1-C3 vector showed efficient transduction both in the TM and corneal endothelium. In anterior segment tissues of scAAV2-eMGP-C3-injected eyes, no obvious morphological changes were found except for the TM. Inflammation was absent. CONCLUSION: In scAAV2-transduced TM cells, the promoter-driven efficiency of Ch3L1 is close to that of cytomegalovirus, but obviously higher than that of MGP. In the anterior chamber of rat eye, the transgene expression pattern of scAAV2 vector is presumably affected by MGP promoter, but not by Ch3L1 promoter. These findings would provide a useful reference for improvement of specificity and safety in glaucoma gene therapy using scAAV2 vector. |
| first_indexed | 2024-03-12T22:00:08Z |
| format | Article |
| id | doaj.art-8772af439d924f33b47576524ad993af |
| institution | Directory Open Access Journal |
| issn | 2222-3959 2227-4898 |
| language | English |
| last_indexed | 2024-03-12T22:00:08Z |
| publishDate | 2023-08-01 |
| publisher | Press of International Journal of Ophthalmology (IJO PRESS) |
| record_format | Article |
| series | International Journal of Ophthalmology |
| spelling | doaj.art-8772af439d924f33b47576524ad993af2023-07-25T08:32:35ZengPress of International Journal of Ophthalmology (IJO PRESS)International Journal of Ophthalmology2222-39592227-48982023-08-011681196120910.18240/ijo.2023.08.0320230803Evaluation of trabecular meshwork-specific promoters in vitro and in vivo using scAAV2 vectors expressing C3 transferaseJun-Kai Tan0Ying Xiao1Guo Liu2Long-Xiang Huang3Wen-Hao Ma4Yan Xia5Xi-Zhen Wang6Xian-Jun Zhu7Su-Ping Cai8Xiao-Bing Wu9Yun Wang10Xu-Yang Liu11Xu-Yang Liu. Xiamen Eye Center, Xiamen University, Xiamen 361004, Fujian Province, China. xliu1213@126.com; Yun Wang. Shenzhen Eye Hospital, Jinan University, Shenzhen Eye Institute, Shenzhen 518040, Guangdong Province, China. s_ywang@126.com; Xiao-Bing Wu. Beijing FivePlus Molecular Medicine Institute Co., Ltd., Beijing 102600, China. wuxb0168@vip.sina.comDepartment of Pathology, Hospital of Chengdu University of Traditional Chinese Medicine, Chengdu 610032, Sichuan Province, ChinaSichuan Provincial Key Laboratory for Human Disease Gene Study, Sichuan Provincial People's Hospital, University of Electronic Science and Technology of China, Chengdu 610072, Sichuan Province, ChinaThe First Affiliated Hospital of Fujian Medical University, Fuzhou 350004, Fujian Province, ChinaBeijing FivePlus Molecular Medicine Institute Co., Ltd., Beijing 102600, ChinaBeijing FivePlus Molecular Medicine Institute Co., Ltd., Beijing 102600, ChinaShenzhen Key Laboratory of Ophthalmology, Shenzhen Eye Hospital, School of Optometry, Jinan University, Shenzhen 518040, Guangdong Province, ChinaSichuan Provincial Key Laboratory for Human Disease Gene Study, Sichuan Provincial People's Hospital, University of Electronic Science and Technology of China, Chengdu 610072, Sichuan Province, ChinaShenzhen Key Laboratory of Ophthalmology, Shenzhen Eye Hospital, School of Optometry, Jinan University, Shenzhen 518040, Guangdong Province, ChinaBeijing FivePlus Molecular Medicine Institute Co., Ltd., Beijing 102600, ChinaShenzhen Key Laboratory of Ophthalmology, Shenzhen Eye Hospital, School of Optometry, Jinan University, Shenzhen 518040, Guangdong Province, ChinaXiamen Eye Center, Xiamen University, Xiamen 361004, Fujian Province, China; Department of Ophthalmology, Shenzhen People's Hospital, the 2nd Clinical Medical College, Jinan University, Shenzhen 518020, Guangdong Province, ChinaAIM: To evaluate the potential of two trabecular meshwork (TM)-specific promoters, Chitinase 3-like 1 (Ch3L1) and matrix gla protein (MGP), for improving specificity and safety in glaucoma gene therapy based on self-complementary AAV2 (scAAV2) vector technologies. METHODS: An scAAV2 vector with C3 transferase (C3) as the reporter gene (scAAV2-C3) was selected. The scAAV2-C3 vectors were driven by Ch3L1 (scAAV2-Ch3L1-C3), MGP (scAAV2-MGP-C3), enhanced MGP (scAAV2-eMGP-C3) and cytomegalovirus (scAAV2-CMV-C3), respectively. The cultured primary human TM cells were treated with each vector at different multiplicities of infections. Changes in cell morphology were observed by phase contrast microscopy. Actin stress fibers and Rho GTPases/Rho-associated protein kinase pathway-related molecules were assessed by immunofluorescence staining, real-time quantitative polymerase chain reaction and Western blot. Each vector was injected intracamerally into the one eye of each rat at low and high doses respectively. In vivo green fluorescence was visualized by a Micron III Retinal Imaging Microscope. Intraocular pressure (IOP) was monitored using a rebound tonometer. Ocular responses were evaluated by slit-lamp microscopy. Ocular histopathology analysis was examined by hematoxylin and eosin staining. RESULTS: In TM cell culture studies, the vector-mediated C3 expression induced morphologic changes, disruption of actin cytoskeleton and reduction of fibronectin expression in TM cells by inhibiting the Rho GTPases/Rho-associated protein kinase signaling pathway. At the same dose, these changes were significant in TM cells treated with scAAV2-CMV-C3 or scAAV2-Ch3L1-C3, but not in cells treated with scAAV2-eMGP-C3 or scAAV2-MGP-C3. At low-injected dose, the IOP was significantly decreased in the scAAV2-Ch3L1-C3-injected eyes but not in scAAV2-MGP-C3-injected and scAAV2-eMGP-C3-injected eyes. At high-injected dose, significant IOP reduction was observed in the scAAV2-eMGP-C3-injected eyes but not in scAAV2-MGP-C3-injected eyes. Similar to scAAV2-CMV-C3, scAAV2-Ch3L1-C3 vector showed efficient transduction both in the TM and corneal endothelium. In anterior segment tissues of scAAV2-eMGP-C3-injected eyes, no obvious morphological changes were found except for the TM. Inflammation was absent. CONCLUSION: In scAAV2-transduced TM cells, the promoter-driven efficiency of Ch3L1 is close to that of cytomegalovirus, but obviously higher than that of MGP. In the anterior chamber of rat eye, the transgene expression pattern of scAAV2 vector is presumably affected by MGP promoter, but not by Ch3L1 promoter. These findings would provide a useful reference for improvement of specificity and safety in glaucoma gene therapy using scAAV2 vector.http://ies.ijo.cn/en_publish/2023/8/20230803.pdfself-complementary aav2chitinase 3-like 1matrix gla proteintrabecular meshworkc3 transferase |
| spellingShingle | Jun-Kai Tan Ying Xiao Guo Liu Long-Xiang Huang Wen-Hao Ma Yan Xia Xi-Zhen Wang Xian-Jun Zhu Su-Ping Cai Xiao-Bing Wu Yun Wang Xu-Yang Liu Evaluation of trabecular meshwork-specific promoters in vitro and in vivo using scAAV2 vectors expressing C3 transferase International Journal of Ophthalmology self-complementary aav2 chitinase 3-like 1 matrix gla protein trabecular meshwork c3 transferase |
| title | Evaluation of trabecular meshwork-specific promoters in vitro and in vivo using scAAV2 vectors expressing C3 transferase |
| title_full | Evaluation of trabecular meshwork-specific promoters in vitro and in vivo using scAAV2 vectors expressing C3 transferase |
| title_fullStr | Evaluation of trabecular meshwork-specific promoters in vitro and in vivo using scAAV2 vectors expressing C3 transferase |
| title_full_unstemmed | Evaluation of trabecular meshwork-specific promoters in vitro and in vivo using scAAV2 vectors expressing C3 transferase |
| title_short | Evaluation of trabecular meshwork-specific promoters in vitro and in vivo using scAAV2 vectors expressing C3 transferase |
| title_sort | evaluation of trabecular meshwork specific promoters in vitro and in vivo using scaav2 vectors expressing c3 transferase |
| topic | self-complementary aav2 chitinase 3-like 1 matrix gla protein trabecular meshwork c3 transferase |
| url | http://ies.ijo.cn/en_publish/2023/8/20230803.pdf |
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