The combination of paeonol, diosmetin-7-O-β-D-glucopyranoside, and 5-hydroxymethylfurfural from Trichosanthis pericarpium alleviates arachidonic acid-induced thrombosis in a zebrafish model
Trichosanthis fruit (TF) is a classic medicinal material obtained from Shandong, China. The peel of this fruit (Trichosanthis pericarpium, TP) is known to exert anti-thrombotic effects. However, the anti-thrombotic active components and mechanisms of TP have yet to be fully elucidated. Combined with...
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| Format: | Article |
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Frontiers Media S.A.
2024-02-01
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| Series: | Frontiers in Pharmacology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fphar.2024.1332468/full |
| _version_ | 1797291904639959040 |
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| author | Shenghua Lin Honglin Ma Shanshan Zhang Wei Fan Chuanlin Shen Jiayu Chen Meng Jin Kun Li Qiuxia He Qiuxia He |
| author_facet | Shenghua Lin Honglin Ma Shanshan Zhang Wei Fan Chuanlin Shen Jiayu Chen Meng Jin Kun Li Qiuxia He Qiuxia He |
| author_sort | Shenghua Lin |
| collection | DOAJ |
| description | Trichosanthis fruit (TF) is a classic medicinal material obtained from Shandong, China. The peel of this fruit (Trichosanthis pericarpium, TP) is known to exert anti-thrombotic effects. However, the anti-thrombotic active components and mechanisms of TP have yet to be fully elucidated. Combined with zebrafish models and high-performance liquid chromatography (HPLC), this study evaluated the endogenous anti-thrombotic effects with the combination of three compounds from TP. First, we used HPLC to investigate the components in the water extract of TP. Next, we used the zebrafish model to investigate the anti-thrombotic activity of the three compound combinations by evaluating a range of indicators. Finally, the expression of related genes was detected by real-time quantitative polymerase chain reaction (qPCR). HPLC detected a total of eight components in TP water extract, with high levels of paeonol (Pae), diosmetin-7-O-β-D-glucopyranoside (diosmetin-7-O-glucoside), and 5-hydroxymethylfurfural (5-HMF). The most significant anti-thrombotic activity was detected when the Pae: diosmetin-7-O-glucoside:5-HMF ratio was 4:3:3. qPCR analysis revealed that the abnormal expression levels of f2, fga, fgb, vwf, ptgs1, and tbxas1 induced by arachidonic acid (AA) were improved. The combination of Pae, diosmetin-7-O-glucoside, and 5-HMF may alleviate AA-induced thrombosis by inhibiting the inflammatory reaction, coagulation cascade reaction, and arachidonic acid metabolism pathways. |
| first_indexed | 2024-03-07T19:43:34Z |
| format | Article |
| id | doaj.art-877768226e9541088b4a8678f8f3523d |
| institution | Directory Open Access Journal |
| issn | 1663-9812 |
| language | English |
| last_indexed | 2024-03-07T19:43:34Z |
| publishDate | 2024-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Pharmacology |
| spelling | doaj.art-877768226e9541088b4a8678f8f3523d2024-02-29T04:49:47ZengFrontiers Media S.A.Frontiers in Pharmacology1663-98122024-02-011510.3389/fphar.2024.13324681332468The combination of paeonol, diosmetin-7-O-β-D-glucopyranoside, and 5-hydroxymethylfurfural from Trichosanthis pericarpium alleviates arachidonic acid-induced thrombosis in a zebrafish modelShenghua Lin0Honglin Ma1Shanshan Zhang2Wei Fan3Chuanlin Shen4Jiayu Chen5Meng Jin6Kun Li7Qiuxia He8Qiuxia He9Biology Institute, Qilu University of Technology (Shandong Academy of Sciences), Jinan, ChinaBiology Institute, Qilu University of Technology (Shandong Academy of Sciences), Jinan, ChinaBiology Institute, Qilu University of Technology (Shandong Academy of Sciences), Jinan, ChinaBiology Institute, Qilu University of Technology (Shandong Academy of Sciences), Jinan, ChinaBiology Institute, Qilu University of Technology (Shandong Academy of Sciences), Jinan, ChinaBiology Institute, Qilu University of Technology (Shandong Academy of Sciences), Jinan, ChinaBiology Institute, Qilu University of Technology (Shandong Academy of Sciences), Jinan, ChinaDepartment of Gastroenterology, The First Affiliated Hospital of Shandong First Medical University and Shandong Provincial Qianfoshan Hospital, Jinan, ChinaBiology Institute, Qilu University of Technology (Shandong Academy of Sciences), Jinan, ChinaScience and Technology Service Platform, Qilu University of Technology (Shandong Academy of Sciences), Jinan, ChinaTrichosanthis fruit (TF) is a classic medicinal material obtained from Shandong, China. The peel of this fruit (Trichosanthis pericarpium, TP) is known to exert anti-thrombotic effects. However, the anti-thrombotic active components and mechanisms of TP have yet to be fully elucidated. Combined with zebrafish models and high-performance liquid chromatography (HPLC), this study evaluated the endogenous anti-thrombotic effects with the combination of three compounds from TP. First, we used HPLC to investigate the components in the water extract of TP. Next, we used the zebrafish model to investigate the anti-thrombotic activity of the three compound combinations by evaluating a range of indicators. Finally, the expression of related genes was detected by real-time quantitative polymerase chain reaction (qPCR). HPLC detected a total of eight components in TP water extract, with high levels of paeonol (Pae), diosmetin-7-O-β-D-glucopyranoside (diosmetin-7-O-glucoside), and 5-hydroxymethylfurfural (5-HMF). The most significant anti-thrombotic activity was detected when the Pae: diosmetin-7-O-glucoside:5-HMF ratio was 4:3:3. qPCR analysis revealed that the abnormal expression levels of f2, fga, fgb, vwf, ptgs1, and tbxas1 induced by arachidonic acid (AA) were improved. The combination of Pae, diosmetin-7-O-glucoside, and 5-HMF may alleviate AA-induced thrombosis by inhibiting the inflammatory reaction, coagulation cascade reaction, and arachidonic acid metabolism pathways.https://www.frontiersin.org/articles/10.3389/fphar.2024.1332468/fullTrichosanthis pericarpiumthrombosisarachidonic acidzebrafishcoagulation cascadeinflammation |
| spellingShingle | Shenghua Lin Honglin Ma Shanshan Zhang Wei Fan Chuanlin Shen Jiayu Chen Meng Jin Kun Li Qiuxia He Qiuxia He The combination of paeonol, diosmetin-7-O-β-D-glucopyranoside, and 5-hydroxymethylfurfural from Trichosanthis pericarpium alleviates arachidonic acid-induced thrombosis in a zebrafish model Frontiers in Pharmacology Trichosanthis pericarpium thrombosis arachidonic acid zebrafish coagulation cascade inflammation |
| title | The combination of paeonol, diosmetin-7-O-β-D-glucopyranoside, and 5-hydroxymethylfurfural from Trichosanthis pericarpium alleviates arachidonic acid-induced thrombosis in a zebrafish model |
| title_full | The combination of paeonol, diosmetin-7-O-β-D-glucopyranoside, and 5-hydroxymethylfurfural from Trichosanthis pericarpium alleviates arachidonic acid-induced thrombosis in a zebrafish model |
| title_fullStr | The combination of paeonol, diosmetin-7-O-β-D-glucopyranoside, and 5-hydroxymethylfurfural from Trichosanthis pericarpium alleviates arachidonic acid-induced thrombosis in a zebrafish model |
| title_full_unstemmed | The combination of paeonol, diosmetin-7-O-β-D-glucopyranoside, and 5-hydroxymethylfurfural from Trichosanthis pericarpium alleviates arachidonic acid-induced thrombosis in a zebrafish model |
| title_short | The combination of paeonol, diosmetin-7-O-β-D-glucopyranoside, and 5-hydroxymethylfurfural from Trichosanthis pericarpium alleviates arachidonic acid-induced thrombosis in a zebrafish model |
| title_sort | combination of paeonol diosmetin 7 o β d glucopyranoside and 5 hydroxymethylfurfural from trichosanthis pericarpium alleviates arachidonic acid induced thrombosis in a zebrafish model |
| topic | Trichosanthis pericarpium thrombosis arachidonic acid zebrafish coagulation cascade inflammation |
| url | https://www.frontiersin.org/articles/10.3389/fphar.2024.1332468/full |
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