A marker-free system for highly efficient construction of vaccinia virus vectors using CRISPR Cas9

A marker-free system for highly efficient construction of vaccinia virus vectors using CRISPR Cas9

The current method for creation of vaccinia virus (VACV) vectors involves using a selection and purification marker, however inclusion of a gene without therapeutic value in the resulting vector is not desirable for clinical use. The Cre-LoxP system has been used to make marker-free Poxviruses, but...

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Bibliographic Details
Main Authors: Ming Yuan, Xuefei Gao, Louisa S Chard, Zarah Ali, Jahangir Ahmed, Yunqing Li, Pentao Liu, Nick R Lemoine, Yaohe Wang
Format: Article
Language:English
Published: Elsevier 2015-01-01
Series:Molecular Therapy: Methods & Clinical Development
Online Access:http://www.sciencedirect.com/science/article/pii/S232905011630047X

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http://www.sciencedirect.com/science/article/pii/S232905011630047X

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