Integrating PCR-free amplification and synergistic sensing for ultrasensitive and rapid CRISPR/Cas12a-based SARS-CoV-2 antigen detection

Integrating PCR-free amplification and synergistic sensing for ultrasensitive and rapid CRISPR/Cas12a-based SARS-CoV-2 antigen detection

Antigen detection provides particularly valuable information for medical diagnoses; however, the current detection methods are less sensitive and accurate than nucleic acid analysis. The combination of CRISPR/Cas12a and aptamers provides a new detection paradigm, but sensitive sensing and stable amp...

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Bibliographic Details
Main Authors: Xiangxiang Zhao, Zhengduo Wang, Bowen Yang, Zilong Li, Yaojun Tong, Yuhai Bi, Zhenghong Li, Xuekui Xia, Xiangyin Chen, Lixin Zhang, Weishan Wang, Gao-Yi Tan
Format: Article
Language:English
Published: KeAi Communications Co., Ltd. 2021-12-01
Series:Synthetic and Systems Biotechnology
Subjects:
CRISPR/Cas12a
Aptamer
Antigen detection
SARS-CoV-2
PCR-Free amplification
Synergistic sensing
Online Access:http://www.sciencedirect.com/science/article/pii/S2405805X21000508

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http://www.sciencedirect.com/science/article/pii/S2405805X21000508

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