Live-cell mapping of organelle-associated RNAs via proximity biotinylation combined with protein-RNA crosslinking

The spatial organization of RNA within cells is a crucial factor influencing a wide range of biological functions throughout all kingdoms of life. However, a general understanding of RNA localization has been hindered by a lack of simple, high-throughput methods for mapping the transcriptomes of sub...

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Bibliographic Details
Main Authors: Pornchai Kaewsapsak, David Michael Shechner, William Mallard, John L Rinn, Alice Y Ting
Format: Article
Language:English
Published: eLife Sciences Publications Ltd 2017-12-01
Series:eLife
Subjects:
Online Access:https://elifesciences.org/articles/29224