The effect of DMSO on ITS2 amplification in the molecular identification of Anopheles farauti Laveran (Diptera: Culicidae), from a colony established in the laboratory

The effect of DMSO on ITS2 amplification in the molecular identification of Anopheles farauti Laveran (Diptera: Culicidae), from a colony established in the laboratory

Sibling species identification is very important in the understanding of malaria epidemiology. Morphological criteria are usually used in the identification of anopheline species, but this fails when sibling or cryptic species occur. Analysis by PCR-RFLP of rDNA ITS2 is currently the most reliable a...

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Bibliographic Details
Main Authors: Ign. Joko Suyono, Jesmandt Situmorang, R.C. Hidayat Soesilohadi, Rarastoeti Pratiwi
Format: Article
Language:English
Published: The Entomological Society of Indonesia 2015-09-01
Series:Jurnal Entomologi Indonesia
Subjects:
molecular identification
sibling species
PCR-RFLP
rDNA ITS2
secondary structure
Online Access:http://jurnal.pei-pusat.org/index.php/jei/article/view/59

Internet

http://jurnal.pei-pusat.org/index.php/jei/article/view/59

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