Antidepressant effects of total alkaloids of Fibraurea recisa on improving corticosterone-induced apoptosis of HT-22 cells and chronic unpredictable mild stress-induced depressive-like behaviour in mice
Context Fibraurea recisa Pierre. (Menispermaceae) (FR) is a traditional Chinese medicine known as “Huangteng.” The total alkaloids of FR (AFR) are the main active ingredients. However, the pharmacological effects of AFR in the treatment of depression have not been reported.Objectives This study inve...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2022-12-01
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| Series: | Pharmaceutical Biology |
| Subjects: | |
| Online Access: | https://www.tandfonline.com/doi/10.1080/13880209.2022.2099429 |
| _version_ | 1811314337139130368 |
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| author | Zhongmei He He Yu Hong Wu Lili Su Kun Shi Yan Zhao Ying Zong Weijia Chen Rui Du |
| author_facet | Zhongmei He He Yu Hong Wu Lili Su Kun Shi Yan Zhao Ying Zong Weijia Chen Rui Du |
| author_sort | Zhongmei He |
| collection | DOAJ |
| description | Context Fibraurea recisa Pierre. (Menispermaceae) (FR) is a traditional Chinese medicine known as “Huangteng.” The total alkaloids of FR (AFR) are the main active ingredients. However, the pharmacological effects of AFR in the treatment of depression have not been reported.Objectives This study investigates the antidepressant effects of AFR by network pharmacology and verification experiments.Materials and methods Compound-Target-Pathway (C-P-T) network of FR and depression was constructed through network pharmacology. In vitro, HT-22 cells were treated with corticosterone (CORT) solution (0.35 mg/mL), then AFR (0.05 mg/mL) solution and inhibitor AZD6244 (14 μM/mL) or BAY11-7082 (10 μM/mL) were added, respectively. The cell viability was detected by CCK-8. In vivo, C57BL/6 mice were divided into 5 groups, namely the normal group, the CUMS group, the AFR (400 mg/kg) group, and the 2 groups that were simultaneously administered the inhibitory group AZD6244 (8 mg/kg) and BAY11-7082 (5 mg/kg). Western blotting was used to assess the expression level of the proteins.Results AFR could protect HT-22 cells from CORT-induced damage and increase the cell viability from 49.12 ± 3.4% to 87.26 ± 1.5%. Moreover, AFR significantly increased the levels of BDNF (1.3, 1.4-fold), p-ERK (1.4, 1.2-fold) and p-CERB (1.6, 1.3-fold), and decreased the levels of NLRP3 (11.3%, 31.6%), ASC (19.2%, 34.2%) and caspase-1 (18.0%, 27.6%) in HT-22 cells and the hippocampus, respectively.Discussion and conclusions AFR can improve depressive-like behaviours and can develop drugs for depression treatment. Further studies are needed to validate its potential in clinical medicine. |
| first_indexed | 2024-04-13T11:10:04Z |
| format | Article |
| id | doaj.art-87b4ac129d824d02b363f71c20fe24a5 |
| institution | Directory Open Access Journal |
| issn | 1388-0209 1744-5116 |
| language | English |
| last_indexed | 2024-04-13T11:10:04Z |
| publishDate | 2022-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Pharmaceutical Biology |
| spelling | doaj.art-87b4ac129d824d02b363f71c20fe24a52022-12-22T02:49:09ZengTaylor & Francis GroupPharmaceutical Biology1388-02091744-51162022-12-016011436144810.1080/13880209.2022.2099429Antidepressant effects of total alkaloids of Fibraurea recisa on improving corticosterone-induced apoptosis of HT-22 cells and chronic unpredictable mild stress-induced depressive-like behaviour in miceZhongmei He0He Yu1Hong Wu2Lili Su3Kun Shi4Yan Zhao5Ying Zong6Weijia Chen7Rui Du8College of Chinese Medicinal Materials, Jilin Agricultural University, Changchun, ChinaCollege of Chinese Medicinal Materials, Jilin Agricultural University, Changchun, ChinaCollege of Chinese Medicinal Materials, Jilin Agricultural University, Changchun, ChinaCollege of Chinese Medicinal Materials, Jilin Agricultural University, Changchun, ChinaCollege of Chinese Medicinal Materials, Jilin Agricultural University, Changchun, ChinaCollege of Chinese Medicinal Materials, Jilin Agricultural University, Changchun, ChinaCollege of Chinese Medicinal Materials, Jilin Agricultural University, Changchun, ChinaCollege of Chinese Medicinal Materials, Jilin Agricultural University, Changchun, ChinaCollege of Chinese Medicinal Materials, Jilin Agricultural University, Changchun, ChinaContext Fibraurea recisa Pierre. (Menispermaceae) (FR) is a traditional Chinese medicine known as “Huangteng.” The total alkaloids of FR (AFR) are the main active ingredients. However, the pharmacological effects of AFR in the treatment of depression have not been reported.Objectives This study investigates the antidepressant effects of AFR by network pharmacology and verification experiments.Materials and methods Compound-Target-Pathway (C-P-T) network of FR and depression was constructed through network pharmacology. In vitro, HT-22 cells were treated with corticosterone (CORT) solution (0.35 mg/mL), then AFR (0.05 mg/mL) solution and inhibitor AZD6244 (14 μM/mL) or BAY11-7082 (10 μM/mL) were added, respectively. The cell viability was detected by CCK-8. In vivo, C57BL/6 mice were divided into 5 groups, namely the normal group, the CUMS group, the AFR (400 mg/kg) group, and the 2 groups that were simultaneously administered the inhibitory group AZD6244 (8 mg/kg) and BAY11-7082 (5 mg/kg). Western blotting was used to assess the expression level of the proteins.Results AFR could protect HT-22 cells from CORT-induced damage and increase the cell viability from 49.12 ± 3.4% to 87.26 ± 1.5%. Moreover, AFR significantly increased the levels of BDNF (1.3, 1.4-fold), p-ERK (1.4, 1.2-fold) and p-CERB (1.6, 1.3-fold), and decreased the levels of NLRP3 (11.3%, 31.6%), ASC (19.2%, 34.2%) and caspase-1 (18.0%, 27.6%) in HT-22 cells and the hippocampus, respectively.Discussion and conclusions AFR can improve depressive-like behaviours and can develop drugs for depression treatment. Further studies are needed to validate its potential in clinical medicine.https://www.tandfonline.com/doi/10.1080/13880209.2022.2099429CUMS modelnetwork pharmacologyBDNF-MEK/ERK signalling pathwayNLRP3/caspase-1 signalling pathway |
| spellingShingle | Zhongmei He He Yu Hong Wu Lili Su Kun Shi Yan Zhao Ying Zong Weijia Chen Rui Du Antidepressant effects of total alkaloids of Fibraurea recisa on improving corticosterone-induced apoptosis of HT-22 cells and chronic unpredictable mild stress-induced depressive-like behaviour in mice Pharmaceutical Biology CUMS model network pharmacology BDNF-MEK/ERK signalling pathway NLRP3/caspase-1 signalling pathway |
| title | Antidepressant effects of total alkaloids of Fibraurea recisa on improving corticosterone-induced apoptosis of HT-22 cells and chronic unpredictable mild stress-induced depressive-like behaviour in mice |
| title_full | Antidepressant effects of total alkaloids of Fibraurea recisa on improving corticosterone-induced apoptosis of HT-22 cells and chronic unpredictable mild stress-induced depressive-like behaviour in mice |
| title_fullStr | Antidepressant effects of total alkaloids of Fibraurea recisa on improving corticosterone-induced apoptosis of HT-22 cells and chronic unpredictable mild stress-induced depressive-like behaviour in mice |
| title_full_unstemmed | Antidepressant effects of total alkaloids of Fibraurea recisa on improving corticosterone-induced apoptosis of HT-22 cells and chronic unpredictable mild stress-induced depressive-like behaviour in mice |
| title_short | Antidepressant effects of total alkaloids of Fibraurea recisa on improving corticosterone-induced apoptosis of HT-22 cells and chronic unpredictable mild stress-induced depressive-like behaviour in mice |
| title_sort | antidepressant effects of total alkaloids of fibraurea recisa on improving corticosterone induced apoptosis of ht 22 cells and chronic unpredictable mild stress induced depressive like behaviour in mice |
| topic | CUMS model network pharmacology BDNF-MEK/ERK signalling pathway NLRP3/caspase-1 signalling pathway |
| url | https://www.tandfonline.com/doi/10.1080/13880209.2022.2099429 |
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