Protocol to isolate human normal and neoplastic pancreatic cells for single-cell omic analyses

Summary: The high-throughput sequencing at single-cell resolution requires high-quality samples of inputted 100–10,000 cells with at least 80%–90% viability according to the applied platform. Here, we present a protocol for single-cell isolation from normal and neoplastic human pancreas. We describe...

Full description

Bibliographic Details
Main Authors: Hao Chen, Junya Peng, Lulu Liu, Dan Huang, Yupei Zhao, Wenming Wu
Format: Article
Language:English
Published: Elsevier 2023-09-01
Series:STAR Protocols
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166723004318