Super-resolution confocal cryo-CLEM with cryo-FIB milling for in situ imaging of Deinococcus radiodurans
Studying bacterial cell envelope architecture with electron microscopy is challenging due to the poor preservation of microbial ultrastructure with traditional methods. Here, we established and validated a super-resolution cryo-correlative light and electron microscopy (cryo-CLEM) method, and combin...
Main Authors: | , , , |
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Format: | Article |
Language: | English |
Published: |
Elsevier
2022-01-01
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Series: | Current Research in Structural Biology |
Subjects: | |
Online Access: | http://www.sciencedirect.com/science/article/pii/S2665928X21000295 |