Super-resolution confocal cryo-CLEM with cryo-FIB milling for in situ imaging of Deinococcus radiodurans

Studying bacterial cell envelope architecture with electron microscopy is challenging due to the poor preservation of microbial ultrastructure with traditional methods. Here, we established and validated a super-resolution cryo-correlative light and electron microscopy (cryo-CLEM) method, and combin...

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Bibliographic Details
Main Authors: Danielle L. Sexton, Steffen Burgold, Andreas Schertel, Elitza I. Tocheva
Format: Article
Language:English
Published: Elsevier 2022-01-01
Series:Current Research in Structural Biology
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S2665928X21000295