4-(Phenylselanyl)-2H-chromen-2-one-Loaded Nanocapsule Suspension—A Promising Breakthrough in Pain Management: Comprehensive Molecular Docking, Formulation Design, and Toxicological and Pharmacological Assessments in Mice
Therapies for the treatment of pain and inflammation continue to pose a global challenge, emphasizing the significant impact of pain on patients’ quality of life. Therefore, this study aimed to investigate the effects of 4-(Phenylselanyl)-2H-chromen-2-one (4-PSCO) on pain-associated proteins through...
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| Format: | Article |
| Language: | English |
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MDPI AG
2024-02-01
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| Series: | Pharmaceutics |
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| Online Access: | https://www.mdpi.com/1999-4923/16/2/269 |
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| author | Caren Aline Ramson da Fonseca Vinicius Costa Prado Jaini Janke Paltian Jean Carlo Kazmierczak Ricardo Frederico Schumacher Marcel Henrique Marcondes Sari Larissa Marafiga Cordeiro Aline Franzen da Silva Felix Alexandre Antunes Soares Robson da Silva Oliboni Cristiane Luchese Letícia Cruz Ethel Antunes Wilhelm |
| author_facet | Caren Aline Ramson da Fonseca Vinicius Costa Prado Jaini Janke Paltian Jean Carlo Kazmierczak Ricardo Frederico Schumacher Marcel Henrique Marcondes Sari Larissa Marafiga Cordeiro Aline Franzen da Silva Felix Alexandre Antunes Soares Robson da Silva Oliboni Cristiane Luchese Letícia Cruz Ethel Antunes Wilhelm |
| author_sort | Caren Aline Ramson da Fonseca |
| collection | DOAJ |
| description | Therapies for the treatment of pain and inflammation continue to pose a global challenge, emphasizing the significant impact of pain on patients’ quality of life. Therefore, this study aimed to investigate the effects of 4-(Phenylselanyl)-2H-chromen-2-one (4-PSCO) on pain-associated proteins through computational molecular docking tests. A new pharmaceutical formulation based on polymeric nanocapsules was developed and characterized. The potential toxicity of 4-PSCO was assessed using <i>Caenorhabditis elegans</i> and <i>Swiss</i> mice, and its pharmacological actions through acute nociception and inflammation tests were also assessed. Our results demonstrated that 4-PSCO, in its free form, exhibited high affinity for the selected receptors, including p38 MAP kinase, peptidyl arginine deiminase type 4, phosphoinositide 3-kinase, Janus kinase 2, toll-like receptor 4, and nuclear factor-kappa β. Both free and nanoencapsulated 4-PSCO showed no toxicity in nematodes and mice. Parameters related to oxidative stress and plasma markers showed no significant change. Both treatments demonstrated antinociceptive and anti-edematogenic effects in the glutamate and hot plate tests. The nanoencapsulated form exhibited a more prolonged effect, reducing mechanical hypersensitivity in an inflammatory pain model. These findings underscore the promising potential of 4-PSCO as an alternative for the development of more effective and safer drugs for the treatment of pain and inflammation. |
| first_indexed | 2024-03-07T22:18:03Z |
| format | Article |
| id | doaj.art-87cdb3b8def844b2b260363439bea0cd |
| institution | Directory Open Access Journal |
| issn | 1999-4923 |
| language | English |
| last_indexed | 2024-03-07T22:18:03Z |
| publishDate | 2024-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pharmaceutics |
| spelling | doaj.art-87cdb3b8def844b2b260363439bea0cd2024-02-23T15:31:16ZengMDPI AGPharmaceutics1999-49232024-02-0116226910.3390/pharmaceutics160202694-(Phenylselanyl)-2H-chromen-2-one-Loaded Nanocapsule Suspension—A Promising Breakthrough in Pain Management: Comprehensive Molecular Docking, Formulation Design, and Toxicological and Pharmacological Assessments in MiceCaren Aline Ramson da Fonseca0Vinicius Costa Prado1Jaini Janke Paltian2Jean Carlo Kazmierczak3Ricardo Frederico Schumacher4Marcel Henrique Marcondes Sari5Larissa Marafiga Cordeiro6Aline Franzen da Silva7Felix Alexandre Antunes Soares8Robson da Silva Oliboni9Cristiane Luchese10Letícia Cruz11Ethel Antunes Wilhelm12Graduate Program in Biochemistry and Bioprospecting, Biochemical Pharmacology Research Laboratory, Federal University of Pelotas, Pelotas CEP 96010-900, RS, BrazilGraduate Program in Pharmaceutical Sciences, Pharmaceutical Technology Laboratory, Federal University of Santa Maria, Santa Maria CEP 97105-900, RS, BrazilGraduate Program in Biochemistry and Bioprospecting, Biochemical Pharmacology Research Laboratory, Federal University of Pelotas, Pelotas CEP 96010-900, RS, BrazilGraduate Program in Chemistry, Chemistry Department, Federal University of Santa Maria, Santa Maria CEP 97105-900, RS, BrazilGraduate Program in Chemistry, Chemistry Department, Federal University of Santa Maria, Santa Maria CEP 97105-900, RS, BrazilClinical Analysis Department, Federal University of Paraná, Curitiba CEP 80210-170, PR, BrazilGraduate Program in Biological Sciences: Toxicological Biochemistry, Federal University of Santa Maria, Santa Maria CEP 97105-900, RS, BrazilGraduate Program in Biological Sciences: Toxicological Biochemistry, Federal University of Santa Maria, Santa Maria CEP 97105-900, RS, BrazilGraduate Program in Biological Sciences: Toxicological Biochemistry, Federal University of Santa Maria, Santa Maria CEP 97105-900, RS, BrazilCenter for Chemical, Pharmaceutical, and Food Sciences, CCQFA, Federal University of Pelotas, Pelotas CEP 96010-900, RS, BrazilGraduate Program in Biochemistry and Bioprospecting, Biochemical Pharmacology Research Laboratory, Federal University of Pelotas, Pelotas CEP 96010-900, RS, BrazilGraduate Program in Pharmaceutical Sciences, Pharmaceutical Technology Laboratory, Federal University of Santa Maria, Santa Maria CEP 97105-900, RS, BrazilGraduate Program in Biochemistry and Bioprospecting, Biochemical Pharmacology Research Laboratory, Federal University of Pelotas, Pelotas CEP 96010-900, RS, BrazilTherapies for the treatment of pain and inflammation continue to pose a global challenge, emphasizing the significant impact of pain on patients’ quality of life. Therefore, this study aimed to investigate the effects of 4-(Phenylselanyl)-2H-chromen-2-one (4-PSCO) on pain-associated proteins through computational molecular docking tests. A new pharmaceutical formulation based on polymeric nanocapsules was developed and characterized. The potential toxicity of 4-PSCO was assessed using <i>Caenorhabditis elegans</i> and <i>Swiss</i> mice, and its pharmacological actions through acute nociception and inflammation tests were also assessed. Our results demonstrated that 4-PSCO, in its free form, exhibited high affinity for the selected receptors, including p38 MAP kinase, peptidyl arginine deiminase type 4, phosphoinositide 3-kinase, Janus kinase 2, toll-like receptor 4, and nuclear factor-kappa β. Both free and nanoencapsulated 4-PSCO showed no toxicity in nematodes and mice. Parameters related to oxidative stress and plasma markers showed no significant change. Both treatments demonstrated antinociceptive and anti-edematogenic effects in the glutamate and hot plate tests. The nanoencapsulated form exhibited a more prolonged effect, reducing mechanical hypersensitivity in an inflammatory pain model. These findings underscore the promising potential of 4-PSCO as an alternative for the development of more effective and safer drugs for the treatment of pain and inflammation.https://www.mdpi.com/1999-4923/16/2/269nanotechnologypain<i>Caenorhabditis elegans</i>miceorganoseleniumcoumarin |
| spellingShingle | Caren Aline Ramson da Fonseca Vinicius Costa Prado Jaini Janke Paltian Jean Carlo Kazmierczak Ricardo Frederico Schumacher Marcel Henrique Marcondes Sari Larissa Marafiga Cordeiro Aline Franzen da Silva Felix Alexandre Antunes Soares Robson da Silva Oliboni Cristiane Luchese Letícia Cruz Ethel Antunes Wilhelm 4-(Phenylselanyl)-2H-chromen-2-one-Loaded Nanocapsule Suspension—A Promising Breakthrough in Pain Management: Comprehensive Molecular Docking, Formulation Design, and Toxicological and Pharmacological Assessments in Mice Pharmaceutics nanotechnology pain <i>Caenorhabditis elegans</i> mice organoselenium coumarin |
| title | 4-(Phenylselanyl)-2H-chromen-2-one-Loaded Nanocapsule Suspension—A Promising Breakthrough in Pain Management: Comprehensive Molecular Docking, Formulation Design, and Toxicological and Pharmacological Assessments in Mice |
| title_full | 4-(Phenylselanyl)-2H-chromen-2-one-Loaded Nanocapsule Suspension—A Promising Breakthrough in Pain Management: Comprehensive Molecular Docking, Formulation Design, and Toxicological and Pharmacological Assessments in Mice |
| title_fullStr | 4-(Phenylselanyl)-2H-chromen-2-one-Loaded Nanocapsule Suspension—A Promising Breakthrough in Pain Management: Comprehensive Molecular Docking, Formulation Design, and Toxicological and Pharmacological Assessments in Mice |
| title_full_unstemmed | 4-(Phenylselanyl)-2H-chromen-2-one-Loaded Nanocapsule Suspension—A Promising Breakthrough in Pain Management: Comprehensive Molecular Docking, Formulation Design, and Toxicological and Pharmacological Assessments in Mice |
| title_short | 4-(Phenylselanyl)-2H-chromen-2-one-Loaded Nanocapsule Suspension—A Promising Breakthrough in Pain Management: Comprehensive Molecular Docking, Formulation Design, and Toxicological and Pharmacological Assessments in Mice |
| title_sort | 4 phenylselanyl 2h chromen 2 one loaded nanocapsule suspension a promising breakthrough in pain management comprehensive molecular docking formulation design and toxicological and pharmacological assessments in mice |
| topic | nanotechnology pain <i>Caenorhabditis elegans</i> mice organoselenium coumarin |
| url | https://www.mdpi.com/1999-4923/16/2/269 |
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