Live slow-frozen human tumor tissues viable for 2D, 3D, ex vivo cultures and single-cell RNAseq

Fresh vs. slow-frozen tissues from various malignancies are compared for the establishment of 2D, 3D and ex vivo cultures, as well as for scRNAseq analysis, and found to be comparable and suitable for cancer research.

Bibliographic Details
Main Authors: Gaetana Restivo, Aizhan Tastanova, Zsolt Balázs, Federica Panebianco, Maren Diepenbruck, Caner Ercan, Bodgan-T. Preca, Jürg Hafner, Walter P. Weber, Christian Kurzeder, Marcus Vetter, Simone Münst Soysal, Christian Beisel, Mohamed Bentires-Alj, Salvatore Piscuoglio, Michael Krauthammer, Mitchell P. Levesque
Format: Article
Language:English
Published: Nature Portfolio 2022-10-01
Series:Communications Biology
Online Access:https://doi.org/10.1038/s42003-022-04025-0