Protocol for assessing phagocytosis activity in cultured primary murine microglia

Summary: In this protocol, we describe steps to assess inflammation-induced cell response in cultured primary murine microglia through the analysis of fluorescent bead phagocytosis. We detail primary murine mixed glial cell culture preparation followed by microglia-specific isolation. Further, we de...

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Bibliographic Details
Main Authors: Elsie Layman, Jennifer Michelle Parrott, Hye Young Lee
Format: Article
Language:English
Published: Elsevier 2022-12-01
Series:STAR Protocols
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166722007614