Improved simultaneous quantitation of candesartan and hydrochlorthiazide in human plasma by UPLCâMS/MS and its application in bioequivalence studies
A validated ultra-performance liquid chromatography mass spectrometric method (UPLCâMS/MS) was used for the simultaneous quantitation of candesartan (CN) and hydrochlorothiazide (HCT) in human plasma. The analysis was performed on UPLCâMS/MS system using turbo ion spray interface. Negative ions were...
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| Format: | Article |
| Language: | English |
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Elsevier
2014-04-01
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| Series: | Journal of Pharmaceutical Analysis |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2095177913000579 |
| _version_ | 1818662768827432960 |
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| author | Bhupinder Singh Rama S. Lokhandae Ashish Dwivedi Sandeep Sharma Naveen Dubey |
| author_facet | Bhupinder Singh Rama S. Lokhandae Ashish Dwivedi Sandeep Sharma Naveen Dubey |
| author_sort | Bhupinder Singh |
| collection | DOAJ |
| description | A validated ultra-performance liquid chromatography mass spectrometric method (UPLCâMS/MS) was used for the simultaneous quantitation of candesartan (CN) and hydrochlorothiazide (HCT) in human plasma. The analysis was performed on UPLCâMS/MS system using turbo ion spray interface. Negative ions were measured in multiple reaction monitoring (MRM) mode. The analytes were extracted using a liquidâliquid extraction (LLE) method by using 0.1 mL of plasma volume. The lower limit of quantitation for CN and HCT was 1.00 ng/mL whereas the upper limit of quantitation was 499.15 ng/mL and 601.61 ng/mL for CN and HCT respectively. CN d4 and HCT-13Cd2 were used as the internal standards for CN and HCT respectively. The chromatography was achieved within 2.0 min run time using a C18 Phenomenex, Gemini NX (100 mmÃ4.6 mm, 5 µm) column with organic mixture:buffer solution (80:20, v/v) at a flow rate of 0.800 mL/min. The method has been successfully applied to establish the bioequivalence of candesartan cilexetil (CNC) and HCT immediate release tablets with reference product in human subjects. Keywords: Candesartan cilexetil, Hydrochlorothiazide, UPLCâMS/MS, Bioequivalence, Candesartan cilexetil-hydrochlorothiazide (ATACAND HCT) |
| first_indexed | 2024-12-17T05:06:12Z |
| format | Article |
| id | doaj.art-87f0dca25ebb48008337523dcbaba8c7 |
| institution | Directory Open Access Journal |
| issn | 2095-1779 |
| language | English |
| last_indexed | 2024-12-17T05:06:12Z |
| publishDate | 2014-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Pharmaceutical Analysis |
| spelling | doaj.art-87f0dca25ebb48008337523dcbaba8c72022-12-21T22:02:26ZengElsevierJournal of Pharmaceutical Analysis2095-17792014-04-0142144152Improved simultaneous quantitation of candesartan and hydrochlorthiazide in human plasma by UPLCâMS/MS and its application in bioequivalence studiesBhupinder Singh0Rama S. Lokhandae1Ashish Dwivedi2Sandeep Sharma3Naveen Dubey4Jubiliant Clinsys Limited, Noida, New Delhi, IndiaJaipur National University, Jaipur, IndiaJubiliant Clinsys Limited, Noida, New Delhi, IndiaJubiliant Clinsys Limited, Noida, New Delhi, IndiaJubiliant Clinsys Limited, Noida, New Delhi, IndiaA validated ultra-performance liquid chromatography mass spectrometric method (UPLCâMS/MS) was used for the simultaneous quantitation of candesartan (CN) and hydrochlorothiazide (HCT) in human plasma. The analysis was performed on UPLCâMS/MS system using turbo ion spray interface. Negative ions were measured in multiple reaction monitoring (MRM) mode. The analytes were extracted using a liquidâliquid extraction (LLE) method by using 0.1 mL of plasma volume. The lower limit of quantitation for CN and HCT was 1.00 ng/mL whereas the upper limit of quantitation was 499.15 ng/mL and 601.61 ng/mL for CN and HCT respectively. CN d4 and HCT-13Cd2 were used as the internal standards for CN and HCT respectively. The chromatography was achieved within 2.0 min run time using a C18 Phenomenex, Gemini NX (100 mmÃ4.6 mm, 5 µm) column with organic mixture:buffer solution (80:20, v/v) at a flow rate of 0.800 mL/min. The method has been successfully applied to establish the bioequivalence of candesartan cilexetil (CNC) and HCT immediate release tablets with reference product in human subjects. Keywords: Candesartan cilexetil, Hydrochlorothiazide, UPLCâMS/MS, Bioequivalence, Candesartan cilexetil-hydrochlorothiazide (ATACAND HCT)http://www.sciencedirect.com/science/article/pii/S2095177913000579 |
| spellingShingle | Bhupinder Singh Rama S. Lokhandae Ashish Dwivedi Sandeep Sharma Naveen Dubey Improved simultaneous quantitation of candesartan and hydrochlorthiazide in human plasma by UPLCâMS/MS and its application in bioequivalence studies Journal of Pharmaceutical Analysis |
| title | Improved simultaneous quantitation of candesartan and hydrochlorthiazide in human plasma by UPLCâMS/MS and its application in bioequivalence studies |
| title_full | Improved simultaneous quantitation of candesartan and hydrochlorthiazide in human plasma by UPLCâMS/MS and its application in bioequivalence studies |
| title_fullStr | Improved simultaneous quantitation of candesartan and hydrochlorthiazide in human plasma by UPLCâMS/MS and its application in bioequivalence studies |
| title_full_unstemmed | Improved simultaneous quantitation of candesartan and hydrochlorthiazide in human plasma by UPLCâMS/MS and its application in bioequivalence studies |
| title_short | Improved simultaneous quantitation of candesartan and hydrochlorthiazide in human plasma by UPLCâMS/MS and its application in bioequivalence studies |
| title_sort | improved simultaneous quantitation of candesartan and hydrochlorthiazide in human plasma by uplcams ms and its application in bioequivalence studies |
| url | http://www.sciencedirect.com/science/article/pii/S2095177913000579 |
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