Generation of human embryonic stem cell-derived lung organoids

Summary: This protocol describes how to generate lung organoids from human embryonic stem cells. Lung organoids form by self-assembly in Matrigel and contain lung epithelial cell types. The protocol presented in this study is simple and only uses 6 cytokines or small molecules. This protocol provide...

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Bibliographic Details
Main Authors: Le Han, Shanshan Zhao, Fuxun Yu, Zhili Rong, Ying Lin, Yong Chen
Format: Article
Language:English
Published: Elsevier 2022-06-01
Series:STAR Protocols
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166722001502