Multi-Layer Controls of Cas9 Activity Coupled With ATP Synthase Over-Expression for Efficient Genome Editing in Streptomyces

Multi-Layer Controls of Cas9 Activity Coupled With ATP Synthase Over-Expression for Efficient Genome Editing in Streptomyces

Efficient genome editing is a prerequisite of genetic engineering in synthetic biology, which has been recently achieved by the powerful CRISPR/Cas9 system. However, the toxicity of Cas9, due to its abundant intracellular expression, has impeded its extensive applications. Here we constructed a gene...

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Bibliographic Details
Main Authors: Kai Wang, Qing-Wei Zhao, Yi-Fan Liu, Chen-Fan Sun, Xin-Ai Chen, Richard Burchmore, Karl Burgess, Yong-Quan Li, Xu-Ming Mao
Format: Article
Language:English
Published: Frontiers Media S.A. 2019-11-01
Series:Frontiers in Bioengineering and Biotechnology
Subjects:
pleiotropic controls
inducible Cas9 activity
homologous recombination
genome editing
Streptomyces
Online Access:https://www.frontiersin.org/article/10.3389/fbioe.2019.00304/full

Internet

https://www.frontiersin.org/article/10.3389/fbioe.2019.00304/full

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