Self-assembled ferritin nanoparticles displaying PcrV and OprI as an adjuvant-free Pseudomonas aeruginosa vaccine
IntroductionSerious infections of Pseudomonas aeruginosa (PA) in hospitals and the emergence and increase of multidrug resistance have raised an urgent need for effective vaccines. However, no vaccine has been approved to date. One possible reason for this is the limited immune response due to the l...
| Main Authors: | , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2023-06-01
|
| Series: | Frontiers in Immunology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fimmu.2023.1184863/full |
| _version_ | 1797798481501356032 |
|---|---|
| author | Yuhang Li Yuhang Li Ruixue Pu Yi Zhang Yiwen Zhang Yujie Wei Sheng Zeng Chen Gao Ying Wang Daijiajia Yin Yueyue Zhang Jiqing Wan Quanming Zou Quanming Zou Jiang Gu |
| author_facet | Yuhang Li Yuhang Li Ruixue Pu Yi Zhang Yiwen Zhang Yujie Wei Sheng Zeng Chen Gao Ying Wang Daijiajia Yin Yueyue Zhang Jiqing Wan Quanming Zou Quanming Zou Jiang Gu |
| author_sort | Yuhang Li |
| collection | DOAJ |
| description | IntroductionSerious infections of Pseudomonas aeruginosa (PA) in hospitals and the emergence and increase of multidrug resistance have raised an urgent need for effective vaccines. However, no vaccine has been approved to date. One possible reason for this is the limited immune response due to the lack of an efficient delivery system. Self-assembled ferritin nanoparticles are good carriers of heterogeneous antigens, which enhance the activation of immunological responses.MethodsIn this study, two well-studied antigen candidates, PcrV and OprI, were selected and connected to the ferritin nanoparticle by the Spytag/SpyCatcher system to generate the nanovaccine rePO-FN.ResultsCompared to recombinant PcrV-OprI formulated with aluminum adjuvants, intramuscular immunization with adjuvant-free rePO-FN induced quick and efficient immunity and conferred protection against PA pneumonia in mice. In addition, intranasal immunization with adjuvant-free rePO-FN enhanced protective mucosal immunity. Moreover, rePO-FN exhibited good biocompatibility and safety.DiscussionOur results suggest that rePO-FN is a promising vaccine candidate, as well as, provide additional evidence for the success of ferritin-based nanovaccines. |
| first_indexed | 2024-03-13T04:04:21Z |
| format | Article |
| id | doaj.art-8882b548d04e40beb0d9a6ce3f81d9dd |
| institution | Directory Open Access Journal |
| issn | 1664-3224 |
| language | English |
| last_indexed | 2024-03-13T04:04:21Z |
| publishDate | 2023-06-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Immunology |
| spelling | doaj.art-8882b548d04e40beb0d9a6ce3f81d9dd2023-06-21T09:44:17ZengFrontiers Media S.A.Frontiers in Immunology1664-32242023-06-011410.3389/fimmu.2023.11848631184863Self-assembled ferritin nanoparticles displaying PcrV and OprI as an adjuvant-free Pseudomonas aeruginosa vaccineYuhang Li0Yuhang Li1Ruixue Pu2Yi Zhang3Yiwen Zhang4Yujie Wei5Sheng Zeng6Chen Gao7Ying Wang8Daijiajia Yin9Yueyue Zhang10Jiqing Wan11Quanming Zou12Quanming Zou13Jiang Gu14College of Pharmacy, Dali University, Dali, ChinaNational Engineering Research Center of Immunological Products, Department of Microbiology and Biochemical Pharmacy, College of Pharmacy, Army Medical University, Chongqing, ChinaThe Third Outpatient Department, The General Hospital of Western Theater Command, Chengdu, ChinaNational Engineering Research Center of Immunological Products, Department of Microbiology and Biochemical Pharmacy, College of Pharmacy, Army Medical University, Chongqing, ChinaNational Engineering Research Center of Immunological Products, Department of Microbiology and Biochemical Pharmacy, College of Pharmacy, Army Medical University, Chongqing, ChinaNational Engineering Research Center of Immunological Products, Department of Microbiology and Biochemical Pharmacy, College of Pharmacy, Army Medical University, Chongqing, ChinaNational Engineering Research Center of Immunological Products, Department of Microbiology and Biochemical Pharmacy, College of Pharmacy, Army Medical University, Chongqing, ChinaNational Engineering Research Center of Immunological Products, Department of Microbiology and Biochemical Pharmacy, College of Pharmacy, Army Medical University, Chongqing, China953th Hospital, Xinqiao Hospital, Army Medical University, Shigatse, ChinaHealth Management Center, PLA Hangzhou Sanatorium, Hangzhou, ChinaNational Engineering Research Center of Immunological Products, Department of Microbiology and Biochemical Pharmacy, College of Pharmacy, Army Medical University, Chongqing, ChinaNational Engineering Research Center of Immunological Products, Department of Microbiology and Biochemical Pharmacy, College of Pharmacy, Army Medical University, Chongqing, ChinaCollege of Pharmacy, Dali University, Dali, ChinaNational Engineering Research Center of Immunological Products, Department of Microbiology and Biochemical Pharmacy, College of Pharmacy, Army Medical University, Chongqing, ChinaNational Engineering Research Center of Immunological Products, Department of Microbiology and Biochemical Pharmacy, College of Pharmacy, Army Medical University, Chongqing, ChinaIntroductionSerious infections of Pseudomonas aeruginosa (PA) in hospitals and the emergence and increase of multidrug resistance have raised an urgent need for effective vaccines. However, no vaccine has been approved to date. One possible reason for this is the limited immune response due to the lack of an efficient delivery system. Self-assembled ferritin nanoparticles are good carriers of heterogeneous antigens, which enhance the activation of immunological responses.MethodsIn this study, two well-studied antigen candidates, PcrV and OprI, were selected and connected to the ferritin nanoparticle by the Spytag/SpyCatcher system to generate the nanovaccine rePO-FN.ResultsCompared to recombinant PcrV-OprI formulated with aluminum adjuvants, intramuscular immunization with adjuvant-free rePO-FN induced quick and efficient immunity and conferred protection against PA pneumonia in mice. In addition, intranasal immunization with adjuvant-free rePO-FN enhanced protective mucosal immunity. Moreover, rePO-FN exhibited good biocompatibility and safety.DiscussionOur results suggest that rePO-FN is a promising vaccine candidate, as well as, provide additional evidence for the success of ferritin-based nanovaccines.https://www.frontiersin.org/articles/10.3389/fimmu.2023.1184863/fullferritin nanoparticlesadjuvant-freePseudomonas aeruginosaPcrVOprIvaccine |
| spellingShingle | Yuhang Li Yuhang Li Ruixue Pu Yi Zhang Yiwen Zhang Yujie Wei Sheng Zeng Chen Gao Ying Wang Daijiajia Yin Yueyue Zhang Jiqing Wan Quanming Zou Quanming Zou Jiang Gu Self-assembled ferritin nanoparticles displaying PcrV and OprI as an adjuvant-free Pseudomonas aeruginosa vaccine Frontiers in Immunology ferritin nanoparticles adjuvant-free Pseudomonas aeruginosa PcrV OprI vaccine |
| title | Self-assembled ferritin nanoparticles displaying PcrV and OprI as an adjuvant-free Pseudomonas aeruginosa vaccine |
| title_full | Self-assembled ferritin nanoparticles displaying PcrV and OprI as an adjuvant-free Pseudomonas aeruginosa vaccine |
| title_fullStr | Self-assembled ferritin nanoparticles displaying PcrV and OprI as an adjuvant-free Pseudomonas aeruginosa vaccine |
| title_full_unstemmed | Self-assembled ferritin nanoparticles displaying PcrV and OprI as an adjuvant-free Pseudomonas aeruginosa vaccine |
| title_short | Self-assembled ferritin nanoparticles displaying PcrV and OprI as an adjuvant-free Pseudomonas aeruginosa vaccine |
| title_sort | self assembled ferritin nanoparticles displaying pcrv and opri as an adjuvant free pseudomonas aeruginosa vaccine |
| topic | ferritin nanoparticles adjuvant-free Pseudomonas aeruginosa PcrV OprI vaccine |
| url | https://www.frontiersin.org/articles/10.3389/fimmu.2023.1184863/full |
| work_keys_str_mv | AT yuhangli selfassembledferritinnanoparticlesdisplayingpcrvandopriasanadjuvantfreepseudomonasaeruginosavaccine AT yuhangli selfassembledferritinnanoparticlesdisplayingpcrvandopriasanadjuvantfreepseudomonasaeruginosavaccine AT ruixuepu selfassembledferritinnanoparticlesdisplayingpcrvandopriasanadjuvantfreepseudomonasaeruginosavaccine AT yizhang selfassembledferritinnanoparticlesdisplayingpcrvandopriasanadjuvantfreepseudomonasaeruginosavaccine AT yiwenzhang selfassembledferritinnanoparticlesdisplayingpcrvandopriasanadjuvantfreepseudomonasaeruginosavaccine AT yujiewei selfassembledferritinnanoparticlesdisplayingpcrvandopriasanadjuvantfreepseudomonasaeruginosavaccine AT shengzeng selfassembledferritinnanoparticlesdisplayingpcrvandopriasanadjuvantfreepseudomonasaeruginosavaccine AT chengao selfassembledferritinnanoparticlesdisplayingpcrvandopriasanadjuvantfreepseudomonasaeruginosavaccine AT yingwang selfassembledferritinnanoparticlesdisplayingpcrvandopriasanadjuvantfreepseudomonasaeruginosavaccine AT daijiajiayin selfassembledferritinnanoparticlesdisplayingpcrvandopriasanadjuvantfreepseudomonasaeruginosavaccine AT yueyuezhang selfassembledferritinnanoparticlesdisplayingpcrvandopriasanadjuvantfreepseudomonasaeruginosavaccine AT jiqingwan selfassembledferritinnanoparticlesdisplayingpcrvandopriasanadjuvantfreepseudomonasaeruginosavaccine AT quanmingzou selfassembledferritinnanoparticlesdisplayingpcrvandopriasanadjuvantfreepseudomonasaeruginosavaccine AT quanmingzou selfassembledferritinnanoparticlesdisplayingpcrvandopriasanadjuvantfreepseudomonasaeruginosavaccine AT jianggu selfassembledferritinnanoparticlesdisplayingpcrvandopriasanadjuvantfreepseudomonasaeruginosavaccine |